微生物学

微生物学

封面介绍：Figure 3. Bacterial cultures and antibiotic treatment. Scale bars： A，2mm； B-F，20μm. A： the bacterial colonies cultured on LB agar for 3 days are round and opaque with neat edges. The surfaces are smooth，and shiny with pale yellow. B： the bacterium cultured with DMEM is motionless，and attached to the floor of culture vessels. C and D show almost no rod-like structures in RAW 264.7 and SH-SY5Y cultures after antibiotic treatment，respectively. However，the cell surface of SH-SY5Y exhibits numerous spine-like structures. E and F are used as controls，showing a large number of rod-like structures in the RAW 264.7 and SH-SY5Y cultures without antibiotics，respectively.

人类及小鼠某些细胞系培养中苯基杆菌的污染

熊天庆，王登莉，谭百宏，等

目的：对细胞培养体系中出现的杆状污染物进行分离和鉴定，并探讨如何清除该污染物.方法：采用固体培养基平板划线法分离细菌株，通过荧光染色和透射电镜对其进行形态学观察；结合16S rRNA基因序列分析，进行菌株鉴定；用生长状态良好的细胞上清复苏冻存的已经污染的细胞，检测细胞复苏的存活率.结果：该污染物经形态学和16S rRNA基因序列鉴定为苯基杆菌.形态学观察表明它有一个二态生命周期：即游动期和附着期.大多数情况下该菌可以与宿主细胞共生，常规抗生素均不能彻底清除该细菌.采用生长状态良好的细胞上清复苏冻存细胞可以明显提高了细胞的存活率.结论：本实验报导了苯基杆菌的二态生命周期，同时我们发现用细胞上清复苏冻存细胞可以显著提高细胞的存活率.

细胞培养；苯基杆菌；污染物

来源出版物：微生物学报，2015，55（2）： 176-186联系邮箱：Yanchao Li，liyanchao@ jlu.edu.cn