复方守宫散对Lewis肺癌小鼠JAK2-STAT3信号通路的影响

沈迪△  李崇慧▲  周晋华  徐海惠 周鹏飞

[摘要] 目的 观察复方守宫散对Lewis肺癌小鼠JAK2-STAT3信号通路的影响。 方法 将55只C57BL/6J小鼠按常规方法接种Lewis肺癌瘤株，6d后选取荷瘤成功的小鼠50只随机分为5组：阳性对照组、西药组、中药高剂量组、中药中剂量组、中药低剂量组，每组10只。阳性对照组：灌服生理盐水0.2mL，1次/d；中药组灌服复方守宫散高剂量组0.6mL（相当于120mg/kg），中剂量组0.4mL（相当于60mg/kg），低剂量组0.2mL，（相当于40g/kg），1次/d；顺铂组按1mg/kg给药，0.1mL/只腹腔注射。每天1次，共14d。在光镜下用HE染色法观察肿瘤组织病理形态学改变情况，用Western Blot法检测JAK2、STAT3蛋白的表达水平。 结果 各给药组JAK2、STAT3蛋白的表达水平出现不同程度降低，且JAK2降低较为明显。 结论 复方守宫散能够抑制肿瘤生长，降低 Lewis瘤细胞中JAK2、STAT3蛋白的表达，并通过此方式阻断JAK2-STAT3信号转导 。

[关键词] 肺癌；信号通路；实验研究

[中图分类号] R734.2   [文献标识码] A   [文章编号] 2095-0616（2014）09-17-05

Study on the effects of Compound Shougong Powder on the JAK2-STAT signaling pathway of mice with Lewis lung carcinoma

SHEN Di1  LI Chonghui2  ZHOU Jinhua2  XU Haihui1  ZHOU Pengfei1

1.Anhui University of Chinese Medicine, Hefei 230000, China; 2.Department of Oncology, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230000, China

[Abstract] Objective To observe the effects of Compound Shougong Powder on the JACK2-STAT3 signaling pathway of mice with Lewis lung carcinoma. Methods 55 C57BL/6J mice were inoculated with tumor cell lines of Lewis lung carcinoma via a conventional method. 50 tumor-bearing mice were selected after six days and assigned to five groups: a positive control group, a western medicine group, a TCM group with high dosages, a TCM group with medium dosages, and a TCM group with low dosages, with 10 mice in each group. The positive control group was orally given normal saline of 0.2 mL once a day; the TCM groups were orally given compound shougong powder of 0.6mL for the group with high dosages (equal to 120 mg/kg), 0.4mL for the group of medium dosages (equal to 60mg/kg), and 0.2 mL for the group with low dosages (equal to 40 mg/kg) once a day; the Western medicine group was intraperitoneally injected with cisplatin of 1 mg/kg, 0.1mL each, once a day for 14 days. Pathological and morphological changes of tumor tissues were observed via HE staining under light microscope, and expression levels of JAK2 and STAT3 proteins were tested via the Western Blot. Results Expression levels of JAK2 and STAT3 proteins in each group decreased with different degrees, and the decrease of JAK2 was relatively significant. Conclusion Compound Shougong Powder inhibits tumor growth, lowers the expression of JAK2 and STAT3 proteins in Lewis tumor cells, and in such way blocks the JACK2-STAT3 signaling pathway.

[Key words] Lung carcinoma; Signaling pathway; Experimental study

肺癌是对人类健康和生命危害最大的恶性肿瘤之一，其中非小细胞肺癌（NSCLC）所占比例高达85％。化疗在中晚期非小细胞肺癌的治疗中发挥着重要作用，但且化疗药物毒副作用较大，使许多中晚期非小细胞肺癌患者难以坚持完成化疗。在

我国，现代医学结合中医药治疗肺癌是一大特色，临床应用有效，本实验将Lewis廇小鼠作为研究体系，观察了中药与西药作用后癌基因STAT3信号通路相关蛋白JAK2 、STAT3 的表达，为中医药的临床运用提供实验依据。

1 材料与方法

1.1 实验动物与细胞

C57BL/6J小鼠50只，体重（20±2） g，雄性，由安徽医科大学二附院动物房提供（动物许可证号：晥20130017 ） 。小鼠Lewis肺癌细胞（ Lewis lung cancer cell line，LLC）， 中国科学院上海生命科学研究院细胞库提供。……