内生真菌—鸭茅互作对宿主植物抗盐性的影响

姬承东 周芸芸

摘要 [目的] 探讨内生真菌对鸭茅植株抗盐性的影响。[方法]以感染内生真菌的鸭茅为试验材料，以无内生菌的鸭茅植株为对照，模拟梯度浓度的盐胁迫环境进行室内盆栽试验，比较带内生真菌E+与不带内生真菌E的鸭茅在相同环境下不同浓度NaCl胁迫下的生长与生理指标变化。[结果] 随盐胁迫浓度的增加， 感染和未感染内生真菌鸭茅植株脯氨酸含量和过氧化物酶活性均显著升高（P<0.05），单株生物量的干、鲜重均显著降低（P<0.05）。与未感染内生菌的鸭茅植株相比，感染内生真菌可使植株的地上生物量和叶片可溶性糖含量显著增加（P<0.05），并使过氧化物酶活性显著降低（P<0.05）。在盐胁迫环境下内生菌对鸭茅叶片中叶绿素和丙二醛的含量无显著影响。[结论]感染内生真菌的鸭茅植株能够增强其对盐胁迫的生理生化适应能力。

关键词 鸭茅；内生真菌；盐胁迫；抗盐性

中图分类号 S432.3+1 文献标识码

A 文章编号 0517-6611（2015）05-109-04

Effects of Interaction between Endophytic Fungi and Dactylis glomerata on the Salt Resistance of Host Plants

JI Chengdong1，2， ZHOU Yunyun1 （1. Hunan Golf and Tourism College， Changde， Hunan 415900； 2. Changsha Hawaii Grass Industry Co. Ltd.， Changsha， Hunan 410200）

Abstract [Objective] The research aimed to discuss the effects of endophytic fungi on the salt resistance of Dactylis glomerata plants. [Method] Taking D. glomerata infected with endophytic fungi as test materials and D. glomerata plants without endophytic fungi as control， the salt stress environment was simulated with different concentration gradients to carry out the indoor pot experiment. The changes of the growth and physiological indices of D. glomerata plants with and without endophytic fungi under the same environment with salt stress with different concentrations of NaCl were compared. [Result] With the increasing of salt concentration， the proline content and peroxidase activity in D. glomerata plants with and without endophytic fungi were significantly increased（P<0.05）， and the dry weight and wet weight of single plant biomass were all significantly decreased（P<0.05）. Compared with D. glomerata plants without endophytic fungi， the aboveground biomass and soluble sugar content in the leaves of D. glomerata plants with endophytic fungi were significantly increased（P<0.05）and their peroxidase activity in D. glomerata plants was significantly decreased（P<0.05）. Under the salt stress environment， the endophytic fungi had no significant effect on the contents of chlorophyll and malondialdehyde in the leaves of D. glomerata. [Conclusion] The physiological and biochemical adaptability of D. glomerata plants infected with endophytic fungi to salt stress was significantly enhanced.

Key words Dactylis glomerata；Endophytic fungi； Salt stress；Salt resistance

基金项目 公益性行业（农业）科研专项（20120304205）。

作者简介 姬承东（1979- ），男，甘肃金昌人，讲师，硕士，从事高尔夫球场草坪养护管理。

收稿日期 20141219

土壤盐渍化是严重影响农业生产和生态环境的问题，在盐胁迫条件下植物的生长缓慢，代谢受到抑制，盐胁迫严重时出现盐斑、叶子萎蔫，甚至死亡[1]。盐渍土在我国有着广泛分布和较大的面积。尤其在新疆地区，盐渍土分布范围廣，改良和发展潜力大，但大多数还未得到合理有效地开发和利用[2-3]。目前，盐渍土的综合利用是旱区和荒漠区农业研究的热点问题，而盐生植物的抗盐性则成为其中的重点之一[2]，对于加快盐渍土的开发利用、促进区域经济发展和盐渍土壤的生态恢复具有重要意义。鸭茅（Dactylis glomerata）又称鸡脚草、果园草，竞争适应能力较强，可与部分豆科草和禾本科草形成稳定、高产的混生群落，适宜放牧、刈割，且通过人工摘顶可以提高人工草地中鸭茅种群的适应性。……