通莲汤对裸小鼠胃癌移植瘤生长及缺氧诱导因子-1α的影响

通莲汤对裸小鼠胃癌移植瘤生长及缺氧诱导因子-1α的影响

张艳丽1，贾永森2，秦丽娟3，孙瑞军3

(1.迁安燕山医院，河北 迁安 064400；2.河北联合大学中医学院，河北 唐山 063000；

3.河北联合大学基础医学院，河北 唐山 063000)

摘要：目的探讨复方通莲汤对胃癌MGC803细胞荷瘤裸鼠的抑瘤作用及对缺氧诱导因子-1α(HIF-1α)表达的影响,为该方临床应用提供实验依据。方法胃癌细胞株MGC803接种于裸鼠右前肢皮下。模型组灌胃生理盐水溶液10 mL/kg，阳性对照组灌胃消癌平片1.8 g(生药量)/kg的药液10 mL/kg, 复方通莲汤高、中、低剂量组分别灌胃1.5，0.75，0.375 g(生药量)/L的药液10 mL/kg，各组连续给药3周，再连续观察3周,6周后处死小鼠，分离瘤组织，计算抑瘤率，免疫组织化学法检测肿瘤组织HIF-1α表达。结果高剂量通莲汤显著抑瘤生长，瘤重(1.51±0.49) g，抑瘤率为(61.04±4.79)%，与模型组比较差异有统计学意义(P<0.05)；通莲汤可显著抑制HIF-1α表达，与模型组比较，阳性表达率χ2=4.286，P=0.038<0.05。结论通莲汤可抑制裸鼠人胃癌MGC803细胞移植瘤生长，其抑瘤机制与下调肿瘤细胞HIF-1α蛋白表达有关。

关键词：通莲汤；胃癌；MGC803细胞；荷瘤裸鼠；缺氧诱导因子-1α

DOI:10.13463/j.cnki.jlzyy.2015.08.025

中图分类号：R285.5文献标志码： A

文章编号：1003-5699(2015)08-0837-04

基金项目:河北省自然科学基金(H2013209053)。

作者简介:张艳丽(1980-)，女，医学硕士，主治医师，主要从事中医内科学研究。

收稿日期:(责任编辑：赵玉芝2014-10-08)

*通信作者:贾永森，电子信箱-jysen@163.com

Tonglian Decoction on tumor growth and HIF-1α expression of tumor tissue

from nude mice modeled with gastric cancer cells

ZHANG Yanli1,JIA Yongsen2,QIN Lijuan3,SUN Ruijun3

(1.Yanshan Hospital of Qian’an,Qian’an,064400,Hebei Province,China;

2.Traditional Chinese Medicine College,Hebei United University,Tangshan 063000,Hebei Province,China;

3.School of Basic Medicine,Hebei United University,Tangshan 063000,Hebei Province,China)

Abstract:ObjectiveThe purpose of the research is to observe inhibition of Tonglian Decoction (TD) on nude mice modeled with gastric cancer cells MGC-803 and on hypoxia inducible factor-1α (HIF-1α) expression in tumor tissue,to provide experimental basis for clinical application of TD.MethodsMGC-803 cells are inoculated subcutaneously in right forelimbs of all mice.Mice in model group are treated with saline solution 10 mL/kg orally.Mice in positive control group are treated with Xiaoaiping tablet,dosage of 10 mL/kg,1.8 g/kg (crude drug) of liquid.Mice in TD groups are subdivided as high (1.5 g/L),medium (0.75 g/L) and low (0.375 g/L) dose group of drug liquid,with dosage of 10 mL/kg.All mice take administration for 3 weeks and are observed for another 3 weeks continuously.Tumor volumes are measured for 6 weeks everyday from the beginning of MGC803 cells transplanted.All mice are killed for isolation of tumor tissue,inhibition ratio of tumor-weight measured.Finally,HIF-1α expression is examined with immunohistochemistry.ResultsTumor weight in TD high-dose group is (1.51±0.49) g,and the inhibition ratio is (61.04±4.79)%,which is significantly different from that in the model group (P<0.05);Positive rate of HIF-1α expression also decreased significantly,showing statistical difference from the model group (χ2=4.286,P=0.038<0.05).ConclusionTD inhibits tumor growth on nude mice modeled with MGC803,through downregulation of HIF-1α protein expression.

Keywords:Tonglian Decoction;gastric cancer;MGC803 cells;nude mice transplanted with tumor;HIF-1α

胃癌是消化系统常见的恶性肿瘤，虽然综合治疗使得胃癌5年生存率有所提高,但患者预后效果仍然较差[1]。研究表明[2]，血管生成与胃癌发生及发展有密切的关系,缺氧诱导因子-1α(HIF-1α)过度表达是促使肿瘤细胞生成、增殖的重要因素之一[3]。HIF-1α作为新近发现的促血管生长因子,可为抗血管生成治疗提供新的作用机制研究。

中医药治疗胃癌具有优势,表现在个体化、多靶点控制肿瘤生长[4]。……