津力达颗粒对1型糖尿病大鼠生长激素-胰岛素样生长因子-1轴的作用

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津力达颗粒对1型糖尿病大鼠生长激素-胰岛素样生长因子-1轴的作用

卢晓晓，王超群，刘子毓，邹俊杰，刘志民*(第二军医大学长征医院内分泌科，上海 200003)

[摘要]目的：探讨津力达颗粒对1型糖尿病大鼠生长激素-胰岛素样生长因子-1(GH/IGF-1)轴的作用。 方法： 采用链脲佐菌素(STZ) 60 mg/kg腹腔注射制备1型糖尿病SD大鼠模型，随机分为7组：糖尿病模型组、低/中/高剂量津力达组、中剂量津力达+通心络组、二甲双胍组、沙格列汀组，另设正常对照组，每组5只。分别予以药物或安慰剂灌胃给药，8周后测定空腹血糖(FBG)、糖化血红蛋白(HbA1C)、空腹胰岛素(FINS)、C肽、生长激素(GH)、胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-1(IGFBP-1)；实时(real-time)测定肝脏生长激素受体(GHR)mRNA。结果：与模型组相比，中、高剂量津力达组的FBG、HbA1C显著降低(P<0.05，P<0.01),FINS、C肽显著升高(P<0.05，P<0.01)；各药物治疗组血清GH显著降低(P<0.01)，IGF-1显著升高(P<0.01)。结论： 津力达颗粒可改善GH/IGF-1轴紊乱。

[关键词]1型糖尿病；津力达颗粒；生长激素；胰岛素样生长因子；大鼠

[中图分类号]R977.15,R965[文献标志码]A

DOI：10.5428/pcar20150206

基金项目国家重点基础研究发展计划(“973”计划，2005CB523304)

作者简介卢晓晓，硕士生. E-mail:1990-lxx@163.com

[收稿日期]2015-01-14

Effects of Jinlida granules on growth hormone/insulin-like growth factor-1 axis in type 1 diabetic rats

LU XiaoXiao，WANG ChaoQun，LIU ZiYu，ZOU JunJie，LIU ZhiMin*(Department of Endocrinology，Changzheng Hospital，Second Military Medical University，Shanghai 200003，China)

ABSTRACT[]Objective：To investigate the effect of Jinlida granules on the regulation of growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis in type 1 diabetic rats. Methods： Type 1 diabetic SD rats were induced by intraperitoneal injection of streptozotocin(STZ) 60 mg/kg. The diabetic rats were randomly divided into 7 groups:the diabetic model group，the low/median/high doses of Jinlida granules groups，the median Jinlida + Tongxinluo group，the metformin group and the saxagliptin group,each consisting of 5 rats. Another 5 normal rats were assigned as control. The rats were administered drugs or placebo by gavage，respectively. After 8 weeks，diabetic parameters were detected，including fasting blood glucose(FBG)，glycosylated hemoglobin Alc (HbA1C)，fasting insulin(FINS)，C peptide，growth hormone(GH),IGF-1，insulin-like growth factor binding protein-1 (IGFBP-1). Hepatic growth hormone receptor(GHR) mRNA was detected by real-time PCR. Results：Compared with the levels of the diabetic model group,the levels of FBG and HbA1C were significantly decreased(P<0.05,P<0.01) in median and high dose Jinlida groups,and the levels of FINS and C peptide were significantly increased(P<0.05,P<0.01). Decreased serum GH levels were also detected in the median and high dose Jinlida groups,as compared with that of the diabetic model group(P<0.01)，and IGF-1 level was increased(P<0.01). Conclusion： Jinlida granules could improve the disorder of GH/IGF-1 axis in diabetic rats.

[KEY WORDS]type 1 diabetes mellitus；Jinlida granules；growth hormone；insulin-like growth factor;rats

[Pharm Care Res，2015，15(2)：103-106]

*通信作者(Corresponding author)：刘志民，E-mail：zmliu_cz@hotmail.com

糖尿病(diabetes mellitus)系胰岛素绝对或相对不足引起的、以慢性高血糖为特征的代谢性疾病。随着糖尿病的进展可引起多系统损伤，体内各激素水平均有不同程度的改变[1]。既往研究表明，糖尿病发生时，循环中生长激素(growth hormone，GH)水平升高，伴胰岛素样生长因子-1(insulin-like growth factor-1，IGF-1)水平降低，即GH/IGF-1轴紊乱[2]，而该轴的紊乱，又会导致胰岛素敏感性降低，血糖水平持续升高[3]。津力达颗粒以人参、桂枝、山药、黄精、苍术、佩兰、葛根等为主药，从中医角度改善水谷精微运化，从而发挥降糖作用[4]。……