黄芪联合应用阿托伐他汀对兔急性心肌梗死不同部位瞬间外向钾电流影响的研究*

实 验 研 究

黄芪联合应用阿托伐他汀对兔急性心肌梗死不同部位瞬间外向钾电流影响的研究*

河北省中医院心内科

张铁军徐颖苗华为董燕平李霞周立颖△王思洲菅鹏辉△△(石家庄 050011)

提要目的：研究黄芪联合应用阿托伐他汀对兔急性心肌梗死(AMI)后梗死边缘区及非梗死区心肌细胞瞬间外向钾电流(Ito)的影响。方法：建立AMI动物模型后，分别通过口服阿托伐他汀、腹腔注射黄芪注射液，酶解分离心室肌细胞,采用全细胞膜片钳记录技术记录梗死边缘区及非梗死区心外膜心肌细胞(Epicardium， Epi) Ito密度。结果：AMI梗死边缘区及非梗死区Epi细胞Ito密度与正常心肌细胞相比存在差异性；阿托伐他汀和黄芪分别使受抑制的梗死边缘区及非梗死区Ito密度恢复；阿托伐他汀和黄芪联合应用使梗死边缘区及非梗死区Ito密度进一步恢复。结论：AMI后梗死边缘区及非梗死区Epi细胞Ito密度存在电生理异常；阿托伐他汀和黄芪对心肌梗死梗死边缘区及非梗死区Ito密度的电生理异常有部分改善作用。

关键词黄芪；阿托伐他汀；心肌梗死；胸痹；真心痛；室性心律失常；膜片钳技术；瞬间外向钾电流

中图分类号：R285.5

文献标识码：码：A

文章编号：号：1007-5615(2015)03-0001-04

AbstractObjective: to study the effect of Huangqi combined with Atorvastatin on moment outward potassium current Itoon epicardium of infarction marginal zone and non-infarction zone in rabbits with AMI. Methods: AMI animal models prepared, Ito density of Epi was recorded with patch clamp technique by taking orally atorvastatin, getting intraperitoneal injection with Huangqi, separating ventricular myocardium through enzymolysis. Results: there was difference between Itodensities of Epi of infarction zone and normal Epi; Atorvastatin and Huangqi could respectively restore the Itodensity of Epi of both zones; the combination could further restore the density. Conclusion: Itodensity of Epi of post-AMI infarction marginal zone and none-infarction zone has electrophysiological abnormality; Atorvastatin and Huangqi can improve the abnormality to a certain degree.

通讯作者：苗华为，男，教授，硕士研究生导师。

Effect of Huangqi Combined with Atorvastatin on Moment Outward

Potassium Current of Different Parts in Rabbits with AMI

Department of Cardiology, Hebei Hospital of Traditional Chinese Medicine

ZHANGTie-junXUYingMIAOHua-weiDONGYan-pingLIXiaHUXue-jing

WANGSi-zhouJIANPeng-hui(Shijiazhuang 050011)

Key wordsHuangqi; Avorvastatin; MI (myocardial infarction); pectoral stuffiness pain; angina pectoris; arrhythmia ventricular; patch clamp technique; moment outward potassium current

植物蒙古黄芪或膜荚黄芪的干燥根，始载于《神农本草经》，为“补药之长”。临床医学观察证实在急性心肌梗死病程中可能出现的室性心律失常是引起心源性猝死主要病因。在急性心肌梗死(AMI)临床治疗方案中，黄芪和他汀类药物常常联合应用，取得较好效果。……