激光微孔脱细胞猪真皮支架对Ⅲ度皮肤烧伤血管化的影响

盛小辉，朱宝昌，冯世海，刘　群，于维陆（.天津医科大学研究生院，天津　00070；.天津市第四医院烧伤科，天津　00；.天津市托福医用原子能科技有限公司，天津　009）



激光微孔脱细胞猪真皮支架对Ⅲ度皮肤烧伤血管化的影响

盛小辉1，朱宝昌1，冯世海2，刘群2，于维陆3
（1.天津医科大学研究生院，天津300070；2.天津市第四医院烧伤科，天津300222；3.天津市托福医用原子能科技有限公司，天津300192）

摘要目的：探讨新的脱细胞方法配合激光打孔制备的激光微孔脱细胞猪真皮支架对大鼠Ⅲ度皮肤烧伤创面血管化的影响。方法：将激光微孔脱细胞猪真皮(A组)、普通打孔脱细胞猪真皮（B组）两种真皮支架分别移植至Ⅲ度皮肤烧伤清创后创面，另设无真皮支架对照创面（C组），1周后加植表皮。大体观察植入真皮支架1、2、3周创面的修复情况，并通过HE染色和免疫组化标记创面中CD31、α-SMA表达阳性血管并计数。结果：制备的脱细胞猪真皮呈瓷白色，有光泽，柔软；显微镜下未见细胞残留。加植表皮后，A组较B组存活率高。两组支架植入后及无支架对照组1～3周创面中CD31、α-SMA表达阳性血管数持续增加，其中两组真皮支架均较无支架组多，差异有统计学意义（P＜0．05）；A组显著高于B、C组（P<0.05）。结论：该方法制备的激光微孔脱细胞猪真皮基质能快速血管化，提高表皮存活率，为一种较理想的真皮替代物。

关键词真皮替代物；脱细胞真皮，猪；微孔；皮肤移植；烧伤；大鼠

Effectoflasermicroporeporcineacellulardemalmatrixonvascularizationofwoundswithfull-thicknessburn

SHENG Xiao-hui1,ZHU Bao-chang1,FENG Shi-hai2,LIU Qun2,YU Wei-lu3
（1.Graduate School,Tianjin Medical University,Tianjin 300070,China;2.Department of Burn,The Fourth Hospital of Tianjin,Tianjin 300222,China;3.Tianjin Toefl Medical Atomic Energy Technology Co.,Ltd,Tianjin 300192,China）

Abstract Objective:To explore the influence of the new method-laser micropore porcine acellular demal matrix in vascularization of wounds in rats with full-thickness burn.Methods：Laser micropore porcine acellular demal matrix(A group)and porcine acellular demal matrix(B group)were respectively transplanted on the rats with full-thickness burn after wound debridement,and there were no dermal in the control group(C group).Then skin was transplanted into the rats of the three groups one week later.The reparation of dermal implant wound was recorded after 1,2 and 3 weeks respectively.At the same time,CD31,α-SMA positive signals detected by immunohistochemical staining and HE staining were used to observe the effect of repairation.Results：The well-prepared laser microspore porcine acellular dermal matrix was characterized by its porcelain white color,softness and good elasticity.Histological examination revealed that laser microspore porcine acellular dermal matrix was totally devoid of epidermis and cellular components.Group A had higher survival rate than group B.From 1th to 3th week,the number of vessels with CD31 and α-SMA expression were continuously increased in the three groups.The number of vessels with CD31 and α-SMA expression in group A and B were significantly higher than in group C(P<0.05).The difference was statistically significant(P<0.05).Conclusion：Laser microspore porcine acellular demal matrix can be used as an ideal dermal substitute due to its ability of initiating revascularization rapidly which may increase the survival rate of the grafted skin.

Key words dermal substitutes;acellular dermal matrix;microporous;skin transplantation;burns；rat

大面积烧伤患者创面多需要进行自体皮肤移植才能修复，但是由于患者的供皮区有限，用皮肤替代物覆盖创面用以保护和促进创面的愈合已成为迫切需要。近年来，异种（猪）脱细胞真皮基质在临床得到了广泛地推广应用[1]。……