不同分子生物学方法检测手足口病标本的应用研究

粘志光河南省郑州大学附属洛阳中心医院检验科，河南洛阳471000

不同分子生物学方法检测手足口病标本的应用研究

粘志光
河南省郑州大学附属洛阳中心医院检验科，河南洛阳471000

目的探究不同分子生物学方法检测手足口病标本的应用。方法选取该院2014年2月—2015年9月采集的209例手足口病标本作为对象进行研究，分别予以巢式PCR、RT-PCR检测，比较两种检测方法检测结果。结果巢式PCR检测CA16阳性率、EV71阳性率及CA16+EV71双阳性率均显著高于RT-PCR检测，差异有统计学意义（P＜0.05）；对比Agilent2100与普通凝胶电泳结果，差异无统计学意义（P＞0.05）；巢式PCR检测对粪便标本、咽拭子标本的检测灵敏度均显著高于RT-PCR检测，差异有统计学意义（P＜0.05）。结论应用巢式PCR检测手足口病标本，其检测灵敏度较高。

分子生物学；检测；手足口病；标本

［Abstract］Objective To study the application of differentmolecular biology methods in detecting hand-foot-mouth dis‐ease.M ethods 209 cases of hand-foot-mouth specimens collected in our hospital from February 2014 to September 2015 were selected as the research objects,and respectively detected by nested PCR and RT-PCR detection,and the detection results of the two methods were compared.Results The positive rates of nested PCR in diagnosis of CA16,EV71 and CA16+EV71 were obviously higher than those of RT-PCR detection,and the differences had statistical significance,P《 0.05,and the difference between the Agilent 2100 result and common gel electrophoresis result had no statistical signifi‐cance,P>0.05,and the detection sensitivity degree of nested PCR for stool specimens and pharynx specimenswas obviously higher than that of RT-PCR detection and the difference had statistical significance,P《0.05.Conclusion The application of nested PCR in detecting hand-foot-mouth disease specimens has a higher detection sensitivity degree.

［Key words］Molecular biology;Detection;Hand-foot-mouth disease;Specimen

手足口病为婴幼儿常见急性感染病，主要由肠道病毒感染导致，其临床主要症状为足底、手部及口腔黏膜疹或溃烂后进展为溃疡，多伴有全身乏力、食欲不振、发热及精神萎靡等[1]。疱疹主要在足心、手部、颊粘膜、牙龈、舌部出现，部分也可在臀部出现。传统病原学诊断方法检测灵敏度较低，价格昂贵，且检测时间较长，无法为临床早期诊治手足口病提供病原学支持[2]。因此，如何建立特异、快速、简便、价格适中的病原学检测方法，从而为手足口病的早期治疗提供有力支持已经成为目前临床学者研究的重要课题[3]。该研究分析不同生物学方法检测在手足口病中的应用，现报道如下。

1　资料与方法

1.1一般资料