辣椒素受体1在功能性消化不良上消化道中的表达及其意义*

史孝敏　彭　燕

西南医科大学附属医院消化内科(646000)



辣椒素受体1在功能性消化不良上消化道中的表达及其意义*

史孝敏彭燕#

西南医科大学附属医院消化内科(646000)

背景：功能性消化不良(FD)是临床上最常见的胃肠疾病之一，严重影响患者的生活质量。目的：探讨辣椒素受体1(TRPV1)在FD患者食管、胃窦、十二指肠黏膜组织中的表达及其意义。方法：选取2012年6月—2014年2月西南医科大学附属医院符合罗马Ⅲ诊断标准的FD患者30例和30例正常对照者，采用免疫组化染色检测食管下段、胃窦部、十二指肠球部黏膜TRPV1、降钙素基因相关肽(CGRP)的表达。结果：FD组食管下段、胃窦部、十二指肠球部黏膜TRPV1、CGRP表达显著高于对照组(P<0.05)。对照组十二指肠球部TRPV1表达显著高于食管下段(P<0.05)，FD组各部位间TRPV1表达差异无统计学意义(P>0.05)。FD组胃窦部CGRP表达显著高于十二指肠球部(P<0.05)，对照组各部位间CGRP表达差异无统计学意义(P>0.05)。对照组TRPV1表达与CGRP表达呈正相关(r=0.443，P<0.05)，FD组TRPV1表达与CGRP表达无关(r=0.277，P>0.05)。结论：FD患者TRPV1在食管下段、胃窦部、十二指肠球部黏膜的表达均较正常人显著增加，可能与其内脏高敏感有关。

辣椒素受体1；降钙素基因相关肽；功能性消化不良；免疫组织化学

Correspondence to:PENG Yan,Email:1806857826@qq.com

Background:Functional dyspepsia (FD) is one of the most common clinical gastrointestinal diseases,and seriously influences the quality of life of patients.Aims:To investigate the expression and significance of transient receptor potential vanilloid type 1 (TRPV1) in esophageal mucosa,gastric antrum mucosa and duodenal mucosa in FD patients.Methods:Thirty patients with FD diagnosed by Rome Ⅲ and 30 health controls from June 2012 to February 2014 at the Affiliated Hospital of Southwest Medical University were enrolled.Expressions of TRPV1 and calcitonin gene-related peptide (CGRP) in lower esophageal,gastric antrum and duodenal bulb mucosa were determined by immunohistochemistry.Results:Expressions of TRPV1 and CGRP in lower esophageal,gastric antrum and duodenal bulb mucosa in FD group were significantly higher than those in control group (P<0.05).Expression of TRPV1 in duodenal bulb mucosa was significantly higher than that in lower esophageal mucosa in control group (P<0.05),however,no significant difference in expression of TRPV1 among different locations in FD group was found (P>0.05).Expression of CGRP in gastric antrum mucosa was significantly higher than that in duodenal bulb mucosa in FD group (P<0.05),however,no significant difference in expression of CGRP among different locations in control group was found (P>0.05).Expression of TRPV1 was positively correlated with expression of CGRP in control group (r=0.443,P<0.05),and expression of TRPV1 was not correlated with expression of CGRP in FD group (r=0.277,P>0.05).Conclusions:The expression of TRPV1 in lower esophageal,gastric antrum and duodenal bulb mucosa in FD is significantly higher than that in normal controls,which might be related to visceral hypersensitivity.

功能性消化不良(functional dyspepsia,FD)是临床上最常见的胃肠疾病之一，其发病机制目前仍不清楚，多数研究认为与内脏高敏感有关[1]。辣椒素受体1(transient receptor potential vanilloid type 1,TRPV1)是一种接受疼痛刺激的感受器，是近年研究镇痛与疼痛的热点分子。FD患者上消化道黏膜中TRPV1的表达及其与内脏高敏感的关系目前尚不清楚。本研究通过检测FD患者食管下段、胃窦和十二指肠球部黏膜中TRPV1表达，旨在探讨FD内脏高敏感的可能机制。

对象与方法

一、研究对象

选择2012年6月—2014年2月西南医科大学附属医院消化内科门诊或胃镜室的FD患者30例，其中男12例，女18例，平均年龄(40.9±4.6)岁。……