不同标本类型对一种NT-proBNP（POCT法）检测结果的影响

龙林会，陈芳四川省自贡市第三人民医院检验科，四川自贡　643000

不同标本类型对一种NT-proBNP（POCT法）检测结果的影响

龙林会，陈芳
四川省自贡市第三人民医院检验科，四川自贡643000

目的 探讨不同标本类型对一种POCT法检测N末端B型利钠肽（NT-proBNP）检测结果的影响。 方法 收集6例患者样本，其中男女各3例。另收集正常体检者2例样本作为样本稀释液。随机收集20例心功能不全病例标本用于相关性分析。结果 以化学发光定量检测作为标准，测定干燥血清管标本的NT-proBNP结果与武汉明德公司POCT法分析仪检测两种标本NT-proBNP浓度的Pearson相关系数分别是r干燥管=0.952 8，rEDTA-K2抗凝管=0.930 7；不同类型标本NT-proBNP（POCT法）浓度比较P＜0.05，差异有统计学意义；将干燥管分离出的血清为最适标本存放在室温下0 h、2 h、4 h、8 h、12 h、24 h后测定的NT-proBNP，各组数据间差异无统计学意义（P＞0.05）。结论 该研究认为采用武汉明德公司POCT法检测NT-proBNP使用干燥真空管采集标本为最适标本。

N末端B型利钠肽，POCT法，检测，心力衰竭

［Abstract］Objective To study the effect of different specimen types on the test results of NT-proBNP by the POCT method.Methods The specimens of 3 female cases and 3 male cases were collected，and another 2 specimens of cases of normal physical examiners were selected as the sample diluent，and 20 specimens of disease cases with cardiac insufficiency were randomly collected for the relevant analysis.Results The Pearson correlation coefficients of NT-proBNP result of dry serum tube specimen and NT-proBNP concentration of two specimens detected by the analysis meter by the POCT method in Wuhan Mingde Company were respectively rdrying tube=0.9528 and rEDTA-K2anticoagulative tube=0.9307 taking the chemiluminescence quantitative test as standards，the difference in the NT-proBNP concentration between different types of specimens had statistical significance，P＜0.05，the serum isolated from the drying tube were used as the optimal specimen and stored at the room temperature，and the NT-proBNP was measured after 0 h，2 h，4 h，8 h，12 h and 24 h，and the difference between groups had no statistical significance，P＞0.05.Conclusion The research shows that the specimen collected by the dry vacuum tube is the optimal specimen after testing NT-proBNP by the POCT method in Wuhan Mingde Company

［Key words］NT-proBNP；POCT method；Detection；Heart failure

心力衰竭（Heart Failure，HF）是指由于心脏功能障碍而产生的体征和症状的临床综合征；它是目前严重危害人类健康的最常见的一种综合征 因此心衰的早期诊断及规范治疗对逆转左室重构，阻断心血管病的发生与发展极为重要B-型脑利钠肽（BNP）主要由心室分泌，因此在诊断心室功能障碍时，是个直接客观的标志物。N末端B型利钠肽（NT-proBNP）和BNP同属利钠肽家族，均用于临床检测心肌细胞受刺激后，产生含134个氨基酸的B型利钠肽原前体（pre-proBNP），随后形成含108个氨基酸的BNP前体（proBNP），后者在内切酶的作用下……