郭尚 李艳婷 周林
摘 要:Fomitiporia yanbeiensis S. Guo & L. Zhou是从山西雁北地区沙棘腐木上分离获得的嗜蓝孢孔菌属的新种,这个种类具有特殊功能成分,具有很高的药用价值。该研究对其菌丝培养条件即温度、pH值、碳源营养、氮源营养进行了试验和分析。結果表明,该菌菌丝体在人工培养基上容易生长,其最适生长温度为25℃,在最适温度下30d左右可长满平皿。最适培养条件:pH为6.0,碳源为葡萄糖,氮源为蛋白胨。这些结果为该菌的人工驯化栽培和功能食品开发提供了参考和依据。
关键词:Fomitiporia yanbeiensis S. Guo & L.Zhou;培养条件;温度;pH值;碳源;氮源
中图分类号 R284 文献标识码 A 文章编号 1007-7731(2017)15-0025-3
Abstract:Fomitiporia yanbeiensis S. Guo & L. Zhou is isolated from the thermophilic fungus spore species of blue hole in Shanxi Yanbei area. This species has a special functional component and has high medicinal value. The mycelial culture conditions, temperature, pH value, carbon source nutrition and nitrogen source nutrition were tested and analyzed. The results showed that the mycelium was easy to grow on the artificial medium, and the optimum growth temperature was 25 ℃, and the culture dish could be filled by mycelium in 30 days at the optimum temperature. The optimum culture pH was 6,carbon source was glucose,and nitrogen source was peptone. These results provide a reference and basis for the domestication cultivation and functional food development of the medicinal fungi.
Key words:Fomitiporia yanbeiensis S. Guo & L. Zhou;Culture conditions; Temperature; pH ; Carbon source; Nitrogen source
Fomitiporia yanbeiensis S. Guo & L. Zhou是一种从山西雁北地区沙棘树分离获得的嗜蓝孢孔菌属真菌,属担子菌门(Basidiomycota)、伞菌纲(Agaricomycetes)、锈革孔菌目(Hymenochaetales)、锈革孔菌科(Hymenochaetaceae)、嗜蓝孢孔菌属(Fomitiporia),子实体蛋白质含量16.10%,粗多糖2.14%,粗脂肪1.02%,氨基酸12.51%,还含有三萜类化合物、葡聚糖、黄酮等多种对人体有益的功能性成分,是一种具有很高开发和利用价值的药食两用菌类[1]。本试验初步研究了Fomitiporia yanbeiensis S. Guo & L. Zhou菌丝体的培养条件,探究了其最适温度、pH值、最佳C源和N源,以期为新菌子实体的培养与开发利用提供技术依据。
1 材料与方法
1.1 菌株 试验菌株材料采集于山西雁北地区,地区海拔1352m;为针阔叶林和混交林地生长地带,寄主为沙棘树(Hippophae rhamnoides Linn.),标本编号SXSYJBB1510,保存于山西省农业科学院食用菌研究所内。以采集的新鲜子实体,进行组织分离得到纯菌株,保存于山西省农科院食用菌所内(SXSYJJZ No.1510)和中国微生物菌种保藏管理委员会普通微生物中心(CGMCC No.13380)。
1.2 培养基……