针刺对自发性高血压大鼠下丘脑室旁核炎症因子含量及Toll样受体4表达的影响

姬彩硕　马思明　杨娜娜　田中雪　杨静雯　邵佳凯　王雪蕊　刘存志

摘要：目的 观察针刺对自发性高血压大鼠（SHR）下丘脑室旁核（PVN）炎症因子含量及Toll样受体4（TLR4）表达的影响，探讨其可能的作用机制。方法 10周龄SHR 30只，随机分为SHR组、针刺组和非穴组，每组10只，另取同周龄WKY大鼠10只为正常组。针刺组针刺双侧“太冲”，采用捻转泻法刺激，非穴组针刺其足背侧，采用平补平泻捻转法刺激，2周后取材。每日检测1次大鼠平均动脉压；RT-PCR检测PVN中TLR4 mRNA的表达；Western blot检测PVN中TLR4蛋白的表达；ELISA检测PVN肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的含量。结果 与正常组比较，SHR组大鼠平均动脉压升高，PVN中TLR4 mRNA和蛋白表达明显升高，TNF-α、IL-6含量明显增加（P<0.05）；与SHR组比较，针刺组大鼠平均动脉压明显下降，PVN中TLR4 mRNA和蛋白表达明显降低，TNF-α、IL-6含量明显减少（P<0.05）；而非穴组无明显变化。结论 针刺“太冲”可能通过抑制SHR PVN中TLR4的表达，减少TNF-α、IL-6分泌，从而降低SHR血压。

关键词：针刺；太冲；高血压；Toll样受体4；炎症因子；自发性高血压大鼠

DOI：10.3969/j.issn.1005-5304.2018.04.013

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）04-0062-04

Abstract： Objective To observe the effects of acupuncture on the level of inflammatory cytokines and toll-like receptor 4 in hypothalamic paraventricular nucleus （PVN） of SHR； To investigate the mechanisms of acupuncture in reduction of mean arterial blood pressure （MAP） in SHR. Methods Thirty 10-week old SHR were randomly divided into SHR group， acupuncture group and non-acupoint group， with 10 rats in each group. 10 WKY rats were set as control group. Acupuncture group received bilateral acupuncture in “Taichong” acupoint， and twisting and diarrhea method was used to stimulate； non-acupoint group received acupuncture at the back of feet， and soothing and diarrhea with twisting method was used to stimulate. Materials were taken two weeks later. The mean arterial pressure of rats was detected every day； the expression of TLR4 mRNA in PVN was detected by RT-PCR； The expression of TLR4 protein in PVN was detected by Western blot； The levels of TNF-αa nd IL-6 were detected. Results Compared with control group， the mean arterial pressure of SHR group increased； TLR4 mRNA and protein expression in PVN increased significantly； the level of TNF-α and IL-6 increased significantly （P<0.05）. Compared with SHR group， the mean arterial pressure of acupuncture group decreased significantly； TLR4 mRNA and protein expression in PVN decreased significantly； the level of TNF-α and IL-6 decreased significantly （P<0.05）. There was no significant change in non-acupoint group. Conclusion Acupuncture in “Taichong” acupoint can attenuate blood pressure of SHR by inhibiting expression of TLR4 in PVN and reducing the levels of TNF-α and IL-6.

Keywords： acupuncture； Taichong； high pressure； TLR4； inflammatory cytokines； spontaneously hypertensive rats

高血壓是一种常见的心脑血管疾病，具有病程长、发展较缓慢的特点。近年来研究表明，高血压发生发展及其靶器官损害与慢性低量炎症反应有关[1]。下丘脑室旁核（hypothalamic paraventricular nucleus，PVN）是脑内最重要的心血管调节中枢，在调节血压中起着重要作用。有研究表明，脑内PVN中促炎因子的增加能够促进高血压的发生发展[2]。Toll样受体家族（toll-like receptors，TLRs）是固有免疫中最主要的组成部分，是炎症信号传递的重要蛋白，能够启动多种与炎症反应相关的基因转录，导致炎症介质的释放，而Toll样受体4（toll-like receptor 4，TLR4）是Toll样受体家族中最主要的蛋白。课题组既往研究发现，针刺“太冲”能有效降低自发性高血压大鼠（SHR）血压[2]。本研究采用RT-PCR、Western blot及ELISA技术，观察针刺“太冲”对SHR PVN中TLR4表达及炎症因子含量的影响，旨在探讨针刺降低血压的可能机制。……