基于网络药理学的吴茱萸抗肿瘤作用机制研究

曾以旺

[摘要] 目的 基于网络药理学研究吴茱萸抗肿瘤的作用机制。 方法 采用活性成分筛选、靶点预测技术，结合生物信息学手段，预测吴茱萸抗肿瘤的潜在作用靶点，并进行信号通路分析，从而探讨其治疗肿瘤的分子机制。 结果 吴茱萸在TCMSP数据中搜索得到30个相应成分，同时利用靶点预测技术共筛选出相关靶点123个。同时在疾病基因数据库检索得到21 365个与肿瘤发生、发展有关的已知靶点基因，利用String数据库和Cytoscape软件中的网络拓扑分析共筛选得出关键靶点5个。 结论 吴茱萸作为中药对肿瘤的作用具有多成分、多靶点、协同作用的特点。

[关键词] 吴茱萸;抗肿瘤;网络药理学;作用靶点;作用机制

[中图分类号] R285          [文献标识码] B          [文章编号] 1673-9701（2020）15-0131-05

Study of anti-tumor mechanism of action of Evodia rutaecarpa based on network pharmacology

ZENG Yiwang

Department of Pharmacy， Ganzhou People's Hospital， Ganzhou   341000， China

[Abstract] Objective To study anti-tumor mechanism of action of Evodia rutaecarpa based on network pharmacology. Methods The potential anti-tumor targets of Evodia rutaecarpa were predicted by active ingredient screening and target prediction technology combined with bioinformatics methods， and the signal pathway analysis was carried out to explore the molecular mechanism of tumor treatment. Results When Evodia rutaecarpa was searched in TCMSP database， 30 corresponding components were found， and 123 related targets were screened by target prediction technology. Meanwhile， 21，365 known target genes related to tumorigenesis and tumor development were retrieved from the disease gene database， and 5 key targets were selected through String database and network topology analysis in the software of Cytoscape. Conclusion As a traditional Chinese medicine， Evodia rutaecarpa has an effect on tumors which is featured multi-component， multi-target and synergistic.

[Key words] Evodia rutaecarpa; Anti-tumor; Network pharmacology; Target of action; Mechanism of action

肿瘤是指机体在各种致瘤因子的作用下，局部组织细胞增生所形成的新生物。近年来， 肿瘤发病率呈高发趋势，病死率逐年上升，已经严重威胁了人类的生命安全。中医对肿瘤早有记载， 宋代之前将生于体内的肿瘤称为癥瘕、积聚等。《诸病源候论》记载：“积聚者，由阴阳不和，脏腑虚弱，受于风邪，搏于脏腑之气所为也”。《景岳全书》提及“脾肾不足及虚弱失调之人，多有积聚之病”。中医认为肿瘤是正气不足、脏腑失调而导致气滞血瘀、痰凝毒聚、毒热蕴结形成肿块，名曰“瘤”、“岩”[1]。目前对于肿瘤的治疗手段主要有手术、化疗、放疗等，化疗药物种类繁多，具有良好的治疗效果，但毒副作用较大，长期应用后肿瘤细胞易产生耐药性。中药是中国传统医学的重要组成部分，许多中药通过多靶点、多途径、多效应治疗肿瘤，同时具有不良反应少、机体耐受性好等优势，在治疗恶性肿瘤方面发挥了重要作用[2]。……