解淀粉芽孢杆菌JL-B05生物学特性及生理生化指标测定

袁宗胜 刘芳 穆景利 周丽丽 黄贞胜 林静 谢宝贵

摘要 [目的]研究解淀粉芽孢杆菌JL-B05的生物学特性及生理生化指标。[方法]对解淀粉芽孢杆菌JL-B05进行平板培养，并对菌体进行革兰氏染色、芽孢染色和荚膜染色，获得解淀粉芽孢杆菌JL-B05的培养特征和形态特征;采用温度梯度和pH梯度法测定解淀粉芽孢杆菌JL-B05的最适培养条件，绘制其40 h内的生长曲线，并对其生理生化指标进行测定。[结果]解淀粉芽孢杆菌JL-B05菌落颜色为白色，菌落边缘不平整，菌体呈杆状，革兰氏阳性，有芽孢，无荚膜;解淀粉芽孢杆菌JL-B05最适培养温度为25 ℃，最佳生长pH为7。[结论]对解淀粉芽孢杆菌JL-B05进行生物学特性及生理生化指标的测定，为后续系统研究解淀粉芽孢杆菌JL-B05及生产复合微生物菌剂奠定基础。

关键词 解淀粉芽孢杆菌;生物学特性;最适培养条件;生理生化指标

中图分类号 Q93 文献标识码 A

文章编号 0517-6611（2020）14-0001-03

doi：10.3969/j.issn.0517-6611.2020.14.001

Abstract [Objective]To study the biological characteristics and physiological & biochemical indexes of Bacillus amyloliquefaciens JL-B05.[Method]The culture characteristics and morphology characteristics of Bacillus amyloliquefaciens JL-B05 were obtained by plate culture，Gram staining，spore staining and capsule staining，and the optimum culture conditions of Bacillus amyloliquefaciens JL-B05 were determined by temperature gradient and pH gradient method，the growth curves of Bacillus amyloliquefaciens JL-B05 were plotted within 40 hours，and their physiological & biochemical indexes were determined.[Result]The colony color of Bacillus amyloliquefaciens JL-B05 was white，the colony edge was uneven，the bacteria were rod-shaped，Gram-positive，there were spores and no capsule.The optimum culture temperature of Bacillus amyloliquefaciens JL-B05 was 25 ℃，the optimum growth pH was 7.[Conclusion]The biological characteristics and physiological & biochemical indexes of Bacillus amyloliquefaciens JL-B05 were determined，which laid the foundation for the subsequent systematic study of Bacillus amyloliquefaciens JL-B05 and the production of compound microbial agents.

Key words Bacillus amyloliquefaciens; Biological characteristics;Optimal culture conditions;Physiological & biochemical indexes

植物內生细菌指其生活史的一定阶段或全部阶段均生活在健康植物的各种组织和器官内部，并且与植物建立了和谐联合关系的细菌[1]。内生细菌通过自身产生代谢产物或借助信号转导对宿主植物生长发育产生重要影响，主要在促进宿主植物生长、增强宿主植物抗性及提高植物修复能力等方面[2-6]，如 Microbacterium 具有促进植物生长的作用[7]， Bacillus具有良好的生防潜力[8]，Sphingomonas 对植物自我修复功能[9]等。笔者选用从毛竹体内分离并筛选出的具有促生、拮抗功能的解淀粉芽孢杆菌JL-B05[10]，研究其生物学特性，即最适生长温度、最适pH、生长曲线及其生理生化指标，为内生解淀粉芽孢杆菌JL-B05的应用及其复合微生物菌剂的开发和利用奠定基础。……