注射用曲札苷对呼吸系统疾病作用的实验研究

赵水仙 杨旭娟 杨晓芸

     【摘 要】 目的：研究注射用曲札茋苷的镇咳、平喘、祛痰及对急性肺损伤治疗效果。方法：分别采用浓氨水致咳法、酚红排泌法、脂多糖致急性肺损伤、豚鼠磷酸组胺和氯化乙酰胆碱引喘法建立相应动物模型，通过观察并记录咳嗽次数、哮喘潜伏期，检测气管酚红排泌量，计算肺指数、肺含水量来探究曲札茋苷对实验动物的影响。结果：与模型组比较，注射用曲札茋苷5 mg/kg能明显减少浓氨水致小鼠咳嗽次数、降低脂多糖诱导小鼠急性肺损伤（Acute Lung Injury，ALI）后的肺指数和肺干湿重比，2.5 mg/kg、5 mg/kg能显著增加小鼠气管酚红排泌量，1.25 mg/kg能明显延长豚鼠哮喘潜伏期。结论：研究表明注射用曲札茋苷具有明显的镇咳、平喘、祛痰及治疗急性肺损伤作用，为其进一步的药用开发提供了实验依据。

【关键词】 注射用曲札茋苷;镇咳;平喘;祛痰;急性肺损伤

【中图分类号】R285.6   【文献标志码】 A    【文章编号】1007-8517（2020）10-0022-05

Abstract：Objective To evaluate the injection with Piceatannol3′-O-glucosideeffect on asthma， cough， acute lung injury. Methods Using the method of strong aqua to cough and phenol red expectorant， LPS induced acute lung injury， guinea pigs and histamine phosphate acetylcholine chloride breath method to establish the corresponding experimental animal model. According to observe and record the cough frequency and asthma attency， detect phenol red excretion of the windpipe， calculate the pulmonary index and water content to explore the effects of Piceatannol3′-O-Glucoside.Results Compared with model group， the injection with Piceatannol3′-O-glucoside 5 mg/kg group can significantly reduce the frequency of concentrated ammonia in mice with cough， reduce the LPS induced acute lung injALIury in mice after lung coefficient and lung wet weight ratio， increase the phenol red excretion in mice tracheal shedding amount， 1.25 mg/kg can obviously prolong the incubation period of asthma guinea pigs.Conclusion The results indicated that injection with Piceatannol3′-O-glucoside has obviously effective on the treatment of cough， asthma， acute lung injury and it improviding powerful evidence for the further development in medical.

Keywords：Injection with Piceatannol3′-O-glucoside; Antitussive Effect; Antiasthmatic Effect; Expectorant Effect; Acute Lung Injury

曲札茋苷是從植物拉萨大黄根茎中提取出来的一种化合物，分子式C20H22O9，属于羟基二苯乙烯类化合物，具有较强的生物活性，具有抗凝血、清除氧自由基、抑制血小板聚集、较强的抗氧化性、保护脑组织、抑制炎症反应和神经细胞凋亡从而阻断对脑组织的损伤作用[1-2]。注射用曲札茋苷为昆药集团股份有限公司自主研发的创新药物，主要用于治疗急性缺血性脑卒中，然而对注射用曲札茋苷用于其他新适应症的报道鲜有，例如对呼吸系统性疾病的作用尚无报道。

咳嗽、哮喘是呼吸系……