烤烟NC82和毕纳1号品种叶片数的遗传模式分析及分子标记筛选

蒲媛媛 郑宏斌 李显航 喻奇伟 熊晶 夏忠文 罗家俊 李星悦 陈谊然 刘仁祥

摘 要：為了提高烤烟品种NC82叶片数改良的效率，本研究选用与NC82叶片数差异显著的毕纳1号分别作为母本和父本，构建了P1、P2、F1、F2遗传研究材料，采用“主基因+多基因”遗传模型分析方法对烤烟叶片数进行了遗传分析，并筛选了烤烟叶片数分子标记。结果表明：烤烟叶片数为非胞质遗传，受2对主效基因与多基因共同影响，最适遗传模型为MX2-EA-AD，主基因+多基因遗传率为92%，主基因和多基因加性效应分别为-3.40、2.37;与叶片数连锁的标记为标记TM20490和TM10102，遗传距离分别是4.8cM和5.3cM。经过试验得知，烤烟叶片数为2对等加性主基因+加性-显性多基因控制的数量性状，主基因遗传率大，采用回交技术结合分子标记辅助选择可定向改良优质烤烟品种NC82的叶片数。

关键词：烤烟;叶片数;遗传分析;SSR分子标记

中图分类号：S572文献标识码：A

文章编号：1008-0457（2020）03-0017-06 国际DOI编码：10.15958/j.cnki.sdnyswxb.2020.03.003

The Genetic Analysig and Molecular Marker Screening of Leaf Number of Flue-cured Tobacco Varieries NC82 and Bina1

PU Yuanyuan.1，ZHENG Hongbin.2，LI Xianhang.1，YU Qiwei.3，XIONG Jing.3，XIA Zhongwen.3，LUO Jiajun.1，LI Xingyue.1，CHEN Yiran.1，LIU Renxiang.1 *

（1. College of Agriculture/Guizhou Key Laboratory of Tobacco Quality Research， Guizhou University， Guiyang， Guizhou 550025， China; 2.China Tobacco Zhejiang Industrial Company， Hangzhou， Zhejiang 310000，China; 3. Bijie Branch of Guizhou Tobacoo Company， Bijie， Guizhou 551700， China）

Abstract：In order to improve the efficiency of improving the leaf number of flue-cured tobacco varieties NC82， this paper selected Bina 1 with a significant difference from the NC82 leaf number as the female and the male parent， respectively， and constructed P1， P2， F1， F2 genetic research materials The "main gene + polygene" genetic model analysis method was used to genetically analyze the leaf number of flue-cured tobacco and screen molecular markers of the leaf number of flue-cured tobacco. The results showed that the leaf number of flue-cured tobacco was non-cytoplasmic， which was affected by two pairs of major genes and polygenes. The optimal genetic model was MX2-EA-AD， and the inheritance rate of major gene+poly gene was 92%， and the additive effects of that were -3.40 and 2.37， respectively. The markers associated with leaf number were TM20490 and TM10102， and the genetic distances were 4.8 cM and 5.3 cM， respectively. In words， the leaf number of flue-cured tobacco was a quantitative trait controlled by 2 equally additive main genes+additive-dominant polygenes， and the main gene has a high heritability. Backcrossing technology combined with molecular marker-assisted selection can be directed to improve the number of leaves of NC82， a high-quality flue-cured tobacco.

Keywords：flue-cured tobacco; leaf number; genetic analysis; SSR molecular markers

烤烟是一种叶用经济作物，叶片数是烟叶产量的主要构成指标之一，叶片数的多少直接影响烟农经济收益和卷烟原料市场的供应[1]。前人研究表明，烤烟叶片数遗传率高[2]，单株叶片数与烟叶产量呈极显著正相关[3-6]，对叶片性状的遗传分析结果表明，烤烟叶片数主要受基因加性效应的影响[7]，同是受多基因共同作用[8-9]。烤烟叶片数加性、显性效应在不同的发育阶段差异明显，其互作效应呈间断性表达[10]。SSR分子标记因均匀覆盖整个基因组，多态性高，操作简单，是常用的提高目……