基于网络药理学的小青龙汤治疗变应性鼻炎作用机制探讨

钟常英 李欣宇 徐慧贤

摘要 目的：通过网络药理学方法探讨小青龙汤治疗变应性鼻炎（Allergic Rhinitis，AR）的作用机制。方法：运用中药系统药理学数据库与分析平台（TCMSP）获得小青龙汤的活性成分及作用靶点，在疾病靶点数据库GeneCards、OMIM获得变应性鼻炎的靶点集，通过R语言将其与中药靶点相映射，从中筛选出共同活性靶点，运用Cytoscape软件、BisoGenet插件构建中药-活性成分-靶点多维网络及小青龙汤-变应性鼻炎蛋白质-蛋白质相互作用（PPI）网络，运用CytoNCA插件、R语言分别进行网络拓扑分析、进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）通路富集分析。结果：获得核心药对“麻黄-甘草”，核心活性成分槲皮素、山柰酚、木犀草素、黄芩素、柚皮素等，核心作用靶点NTRK1、MCM2、CUL3、ESR1、TP53等，涉及通路为AGE-RAGE信号通路、KSHV、TNF信号通路、人巨细胞病毒感染、乙肝、IL-17信号通路、Th17细胞分化等。结论：小青龙汤可通过干预AGE-RAGE等信号通路抑制核因子κB核基因表调节基因转录，通过介导IL-17细胞因子调节Th17细胞分化进一步调节Th1/Th2平衡达到抗炎、调节免疫作用。

关键词 小青龙汤;变应性鼻炎;网络药理学;作用机制

Abstract Objective：To discuss the mechanism of Xiaoqinglong Decoction （XQL Decoction） in the treatment of allergic rhinitis by network pharmacology.Methods：The ingredients targets of XQL Decoction were acquired from Traditional Chinese Medicine Systems Pharmacology Databaseand Analysis Platform（TCMSP），AR targets were obtained from GeneCards and OMIM database.Then the AR targets and ingredients targets were mutual mapped to find the commontargets，Cytoscape（3.7.1） was used to establish herb-ingredients-targets network and protein-protein interaction network（ppi） and network topology analysis R version was used to gain the GO and KEGG enrichment analysis.Results：Ephedra-liquorice is core herb pair; core ingredients are quercetin，kaempferol，luteolin，baicalein，naringenin，etc.Core targets are NTRK1，MCM2，CUL3，ESR1，TP53，UBC，etc.AGE-RAGE，KSHV，TNF signaling pathway，Hu-man cytomegalovirus infection，hepatitis B，IL-17 signaling pathway，Th17 cell differentiation are involved.Conclusion：XQL Decoction can inhibit NF-κB nuclear gene table regulation gene transcription by interfering with AGE-RAGE and other signaling pathways，and regulate Th1/Th2 balance by mediating IL-17 cytokines to regulate Th17 cell differentiation to achieve anti-inflammatory and immune regulation effects.

Keywords Xiaoqinglong Decoction; Allergic Rhinitis; Network Pharmacoloy; Machanism

中圖分类号：R285文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.22.008

变应性鼻炎（Allergic Rhinitis，AR）是临床最常见疾病之一，其发病率逐年上升，且日渐年轻化，目前全球患病人数已达5亿，对患者的生命质量、精神压力、经济负担均带来很大影响[1]。此外，AR常导致多种并发症，如哮喘、鼻-鼻窦炎、分泌性中耳炎、过敏性结膜炎等。目前西医治疗AR主要是药物治疗及免疫治疗，药物治疗易出现鼻部及嗅觉障碍不良反应，免疫治疗局限性较大，而中医药治疗AR的经验古有之，临床实践疗效可靠，具有独特优势。……