碳点促进作物种子萌发及生长的机制研究

2021-01-05 13:14:01刘振玲李亚伟杨涵越刘晓飞王传洗
安徽农业科学 2021年24期

刘振玲 李亚伟 杨涵越 刘晓飞 王传洗

摘要 [目的]研究碳点(CDs)对作物种子萌发及生长的影响。[方法]采用微宇宙培养系统,探究10 mg/L下CDs处理对菠菜(伏播611)种子发芽性能及幼苗生长的影响。[结果]在菠菜种子培植10 d后,CDs处理的菠菜种子萌发率为69.0%,显著高于对照组(46.0%,P<0.05)。CDs增加了菠菜幼苗的生物量,相对于对照组,鲜重提高30.1%,干重提高55.3%。CDs上调了菠菜种子的水通道蛋白基因表达,增加对水分的吸收,促进菠菜种子萌发及生长。[结论]CDs可以作为一种优良的纳米材料应用于现代农业发展中,特别是应用在作物种子的萌发及生长方面。

关键词 碳点;菠菜种子;萌发;幼苗生长;水通道蛋白基因

中图分类号 Q945  文献标识码 A

文章编号 0517-6611(2021)24-0001-05

doi:10.3969/j.issn.0517-6611.2021.24.001

Study on the Mechanism of Carbon Dots Promoting Crop Seed Germination and Growth

LIU Zhen-ling1,LI Ya-wei1,YANG Han-yue2 et al (1.Wuxi New Vision Environmental Protection Co.,Ltd.,Wuxi,Jiangsu 214135;2.Institute of Environmental Processes and Pollution Control,School of Environment and Civil Engineering,Jiangnan University,Wuxi,Jiangsu 214122)

Abstract [Objective]To study the effects of carbon dots (CDs) on the germination and growth of crop seeds.[Method]A microcosmic culture system was used to explore the effects of CDs (10 mg/L) on the germination performance and seedling growth of spinach (Fubo 611).[Result]After the spinach seeds were cultivated for 10 days,the seeds germination rate treated with CDs was 69.0%,which was significantly higher than that of the control group (46.0%,P<0.05).CDs increased the biomass of spinach seedlings relative to the control group (fresh and dry weight increased by 30.1% and 55.3%,respectively).CDs up-regulated the aquaporin gene expression of spinach seeds,increased water absorption,and promoted the germination and growth of spinach seeds.[Conclusion]CDs can be used as excellent nanomaterials for developing modern agriculture,especially in the germination and growth of crop seeds.

Key words Carbon dot;Spinach seed;Germination;Seedling growth;Aquaporin gene

基金项目 中央高校基本科研业务费专项资金(JUSRP221023)。

作者简介 刘振玲(1981—),女,江苏徐州人,高级工程师,硕士,从事环境评估和治理研究。*通信作者,研究员,博士,博士生导师,从事环境功能材料研究。

收稿日期 2021-04-14

我國是一个农业大国,长期面临着人多地少、资源短缺、粮食不足的压力。农业生产过多依赖资源消耗,农业环境污染问题日益突出。因此,如何探索出一条高效、安全、资源节约、环境友好的可持续农业发展道路,是当前必须应对的重大挑战。近年来,以人工纳米材料(engineered nanomaterials,ENMs)生产的纳米化肥、纳米农药、纳米农业传感器等新颖纳米农业产品,在提高作物产量和品质、降低资源投入、减少农业环境污染等方面已经显现出巨大潜力[1-3]。已有研究表明,250  mg/kg CeO2 ENMs 在土壤中能够抑制……

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