高效絮凝菌的分离鉴定及其絮凝条件的优化

林映津 陈倩 曾小妹 谢贻冬 付洋

摘要 采用微生物分离纯化技术从底泥样品中筛选到一株絮凝菌，通过目标菌株的形态学特征和16S rRNA序列分析以鉴定其种屬，并对其发酵条件和絮凝条件进行优化。结果表明，XN1-4为黏质沙雷氏菌;XN1-4在发酵28 h后其发酵液的絮凝效果最好。菌株XN1-4的最佳培养条件为培养基初始pH 5.0、培养温度25  ℃、摇床转速150 r/min;最佳絮凝条件为发酵液投加量4%、助凝剂投加量4%。在最佳絮凝条件下，XN1-4的絮凝率达到98%，且絮凝活性成分主要存在于菌体本身。

关键词 絮凝菌;分离鉴定;培养条件;絮凝条件;优化

中图分类号 X52  文献标识码 A

文章编号 0517-6611（2021）24-0006-05

doi：10.3969/j.issn.0517-6611.2021.24.002

Isolation and Identification of High-efficiency Flocculating Bacteria and Optimization of Flocculation Conditions

LIN Ying-jin1，2，CHEN Qian1，ZENG Xiao-mei1，2 et al （1.Huachuan Technology Co.，Ltd.，Fuzhou，Fujian 350008;2.Fujian Engineering and Research Center of Ecological Restoration of Lake，Reseavoir & River Basin，Fuzhou，Fujian 350008）

Abstract Using microbial separation and purification technology，a flocculating bacteria was isolated from the sediment samples.The bacterial species were identified characterized based on morphological characteristics and 16S rRNA sequence analysis，and its fermentation and flocculation conditions were optimized.The results showed that the flocculating bacteria XN1-4 belonged to Serratia marcescens; strain XN1-4 had the best flocculation effect in the fermentation broth after cultured for 28 h.The optimal culture conditions of strain XN1-4 were the initial pH of the medium 5.0，the culture temperature of 25  ℃，and the rotating speed of the shaker 150 r/min;the optimal flocculation conditions were the fermentation broth dosage of 4% and the coagulant dosage of 4%.Under the optimal flocculation conditions，the flocculation rate of XN1-4 reached 98%，and the flocculating active components mainly existed in the bacteria itself.

Key words Flocculating bacteria;Isolation and identification;Culture conditions;Flocculation conditions;Optimization

基金项目 福州市科技计划项目（2019-S-110）。

作者简介 林映津（1965—），男，福建福州人，工程师，从事水污染生态与修复方面研究。

收稿日期 2021-04-20

随着社会经济的快速发展，人们生活水平的不断提高，日益凸显的河湖生态环境问题也越来越受到关注和重视。虽然，目前也采取了许多截污措施，但由于人口及工业的快速发展，水体富营养化等水生态环境问题仍然日益严重[1-3]，许多地方的河流及湖泊均出现了不同程度的水体富营养化现象，藻类大量繁殖、水体透明度和溶解氧都明显降低[4]，不仅严重影响了水体生态系统，而且严重危害了人类的身心健康[5]。因此，高效修复富营养化等被破坏的水体成为亟待解决的生态环境问题。然而，目前对富营养化等受到破坏的水体多采用物理或化学等方法，效果并不十分理想，并且容易产生二次污染等问题，因此，迫切需要寻找一种高效且不产生二次污染等问题的方法。……