盐胁迫对紫丹参生长及生理特性的影响

崔卓越 徐皓 田爱梅

摘要 分别用0、0.3%、0.5%、0.7%、0.9% NaCl溶液处理紫丹参种子及幼苗，对不同浓度盐胁迫下紫丹参种子的萌发率和幼苗生长状况及生理特性进行研究。结果表明，随着盐浓度的逐渐增加，紫丹参种子的发芽速度和发芽率均呈减小趋势，幼苗期主根及侧根生长减缓，可溶性糖、可溶性蛋白及丙二醛含量逐渐上升。在高盐胁迫下幼苗出现盐害症状，植株叶片变黄脱落，总丹参酮含量先增加后降低，总酚酸含量呈下降趋势，叶绿素含量也随盐胁迫浓度的增加逐渐降低，甚至导致植株死亡。盐胁迫下，紫丹参的生长状况呈下降趋势，土壤中盐分含量过高，使植物生理代谢紊乱，出现盐害现象。

关键词 紫丹参;盐胁迫;抗盐性;NaCl溶液;叶绿素含量

中图分类号 S567.5+3  文献标识码 A

文章编号 0517-6611（2021）24-0070-05

doi：10.3969/j.issn.0517-6611.2021.24.016

Effects of Salt Stress on Growth and Physiological Characteristics of Salvia Miltiorrhiza Bunge

CUI Zhuo-yue1，2，XU Hao1，TIAN Ai-mei2

（1.Shaanxi University of Technology，Hanzhong， Shaanxi  723001;2.Xi’an University，Xi’an ，Shaanxi 710065）

Abstract The seeds and seedlings of Salvia miltiorrhiza Bunge were treated with 0，0.3%，0.5%，0.7% and 0.9% NaCl （mmol / L）， respectively.The germination rate of seeds， growth status and physiological characteristics of seedlings were studied under different concentrations of salt stress.With the increase of salt concentration， the germination and germination rate of Salvia miltiorrhiza seeds decreased， the growth of main root and lateral root slowed down， and the contents of soluble sugar， soluble protein and malondialdehyde increased gradually.Under high salt stress， the seedlings showed symptoms of salt damage， the leaves turned yellow and fell off， the total tanshinone content increased first and then decreased， the total phenolic acid content showed a downward trend， and the chlorophyll content gradually decreased with the increase of salt stress， even leading to plant death.Under salt stress， the growth of Salvia miltiorrhiza showed a downward trend， and the salt content in soil was too high， which led to the disorder of plant physiological metabolism and salt damage.

Key words Salvia miltiorrhiza Bunge;Salt stress;Salt resistance;NaCl solution;Chlorophyll content

作者簡介 崔卓越（1995—），女，陕西榆林人，硕士研究生，研究方向：植物生殖发育。*通信作者，教授，博士，从事植物生殖发育研究。

收稿日期 2021-03-24

土壤盐渍化是我国农业发展的主要问题之一，因土壤底层的盐分随水分上升至地表，水分蒸发致使大量盐分在地表板结。土壤中盐分积累过高使植物生长减缓、代谢受到抑制、光合作用降低等，严重的会导致植物死亡[1]。紫丹参作为一种重要的中药材，药用部位主要是根和根茎，土壤环境对紫丹参根系生长有重要的作用。因而深入探讨不同浓度盐胁迫条件下紫丹参的生长状况具有重要的理论和实践意义。紫丹参（Salvia miltiorrhiza Bunge）又名红根、赤参、丹参等，为唇形科多年生草本植物。……