山羊ADCY1和CYM基因多态性及其与产羔数关联分析

李昆谕 洪琼花 陶林 江炎庭 欧阳依娜 储明星 刘玉芳

摘要 [目的]分析腺苷酸環化酶1（ADCY1）和胰凝乳蛋白酶（CYM）基因单核苷酸多态性（SNPs）与山羊产羔性状的关联。[方法]通过 MassARRAYSNP分型技术对ADCY1和CYM基因SNPs位点进行分型，检测其在云上黑山羊（n=544）、济宁青山羊（n=133）和辽宁绒山羊（n=91）3个群体中的遗传学特征，并将其多态性与产羔性能（产羔数、初生窝重、断奶窝重）进行关联分析。[结果]群体遗传学结果表明，ADCY1基因4个突变在云上黑山羊、济宁青山羊、辽宁绒山羊中均为低度多态（PIC≤0.25）;CYM基因g.87624254G>A和g.87627082G>A 2个位点在3种山羊中均为中度多态（0.25<PIC<0.50）;卡方检验显示，除ADCY1 g.43996583C>T在辽宁绒山羊中处于Hardy-Weinberg不平衡状态（P<0.05），ADCY1基因其余位点和CYM基因g.87624269 G>A、g.87627082G>A 2个位点在3个群体中均处于Hardy-Weinberg平衡状态（P>0.05）。关联分析结果表明，在云上黑山羊中ADCY1基因g.43979904G>A、g.43974472G>A，CYM基因g.87624254G>A位点多态性与产羔数存在显著关联（P<0.05）;ADCY1基因g.43996550G>A、g.43996583C>T，CYM基因g.87624254G>A、g.87627082G>A位点多态性与初生窝重存在显著关联（P<0.05）。[结论]ADCY1基因g.43974472G>A和g.43979904G>A位点可作为云上黑山羊产羔数选择的潜在分子标记。ADCY1基因g.43996550G>A、g.43996583C>T位点，CYM基因g.87627082G>A位点可作为云上黑山羊初生窝重选择的潜在分子标记;CYM基因g.87624254G>A位点可作为云上黑山羊窝重选择的潜在分子标记。

关键词 山羊;产羔数;窝重;ADCY1基因;CYM基因

中图分类号 S827  文献标识码 A  文章编号 0517-6611（2021）24-0141-05

doi：10.3969/j.issn.0517-6611.2021.24.033

Polymorphism of Goat ADCY1 and CYM and Its Association with Litter Size

LI Kun-yu1， 2， HONG Qiong-hua3， TAO Lin2 et al

（1. College of Life Science and Food Engineering， Hebei University of Engineering， Handan， Hebei 056001;2. Key Laboratory of Animal Genetics， Breeding and Reproduction of Ministry of Agriculture and Rural Affairs， Institute of Animal Science， Chinese Academy of Agricultural Sciences， Beijing 100193;3.Yunnan Animal Science and Veterinary Institute， Kunming， Yunnan 650224）

Abstract [Objective]This study aimed to analyze the association between single nucleotide polymorphisms （SNPs） in the adenylate cyclase 1 （ADCY1） and chymotrypsin （CYM） genes and kidding performance in goats.[Method]MassARRAYSNP typing technology was applied to genotype the SNPs of ADCY1 and CYM genes and detect their genetic characteristics in Yunshang black goat （n=544）， Jining grey goat （n=133） and Liaoning cashmere goat （n=91）. Then the association was analyzed between ADCY1 and CYM and kidding performance （litter size， litter weight at birth， litter weight at weaning） in different goats. [Result]The results of population genetics showed that the 4 mutations of ADCY1 gene were low polymorphisms in Yunshang black goat， Jining grey goat， and Liaoning cashmere goat （PIC≤0.25）;CYM gene g.87624254G>A and g.87627082G>A both candidate locus were moderately polymorphic in the three goats （0.25<PIC<0.5）. Chi-square test showed that， except ADCY1 g.43996583C>T in Liaoning cashmere goats， which is in Hardy-Weinberg imbalance （P<0.05）， the other locus are in Hardy-Weinberg equilibrium （P>0.05）;CYM gene g.87624269 two locus G>A and g.87627082G>A were in Hardy-Weinberg equilibrium in the three populations （P>0.05）. The results of association analysis showed that in Yunshang black goats， the ADCY1 gene g.43974472G>A， g.43979904G>A， and CYM gene g.87624254G>A polymorphisms were significantly associated with litter size （P<0.05）;ADCY1 gene g.43996550G>A， g.43996583C>T， CYM gene g.87624254G>A， g.87627082G>A polymorphisms were significantly associated with the primary litter weight （P<0.05）. [Conclusion]In summary， ADCY1 gene g.43974472G>A and g.43979904G>Acan be used as a potential molecular marker for litter size in Yunshang black goats.ADCY1 geneg.43996550G>A and g.43996583C>T locus， CYM gene g.87627082G>A locus can be used as potential molecular markers for litter weight at birth in Yunshang black goats; CYM gene g.87624254G>A locus can be used as potential molecular markers for litter weight selection in Yunshang black goats.

Key words Goat;Litter size;Litter weight;ADCY1 gene;CYM gene

基金项目 国家肉羊产业技术体系专项（CARS-38）;河北省自然科学基金青年项目（C2019402261）;云南省科技创新人才计划（2018HC017）;云南省万人计划——产业技术领军人才项目;中国农业科学院科技创新工程（CAAS-ZDRW202106和ASTIP-IAS13）。

作者简介 李昆谕（1996—），男，甘肃定西人，硕士研究生，研究方向：动物遗传育种;洪琼花（1968—），女，云南鹤庆人，研究员，从事羊遗传育种研究。……