NaCl协同高压电场提高紫花苜蓿辐射敏感性的研究

徐凯迪 陈浩 宋智青 丁昌江

摘要 以紫花苜蓿为诱变材料，通过研究NaCl和高压电场的协同作用，探究高压电场诱变紫花苜蓿的有效方法。结果表明，经过NaCl和高压电场的协同处理后，存活率达到对照组的50%以下，处理组紫花苜蓿的茎长、根长、苗高和鲜重都比对照组低，活性氧（ROS）含量较对照组显著升高。结果表明，NaCl能够提高电场对紫花苜蓿的辐射敏感性。同时，为研究高压电场中的非均匀电场和离子风的各自贡献，利用高压电场加遮挡处理紫花苜蓿，结果证明，在NaCl与高压电场的协同作用中，离子风起了重要的作用。该研究找到NaCl与高压电场联合处理提高紫花苜蓿致死率的方法，并为紫花苜蓿的诱变工作提供了新方法，为高压电场诱变技术更广泛的应用提供了试验基础。

关键词 NaCl;高压电场;紫花苜蓿;辐射敏感性

中图分类号 S541+.1  文献标识码 A

文章编号 0517-6611（2021）04-0001-04

doi：10.3969/j.issn.0517-6611.2021.04.001

Study of NaCl Cooperating with High Voltage Electric Field to Improve Radiosensitivity of Alfalfa

XU Kai-di，CHEN Hao，SONG Zhi-qing et al （College of Science，Inner Mongolia University of Technology，Hohhot，Inner Mongolia 010051）

Abstract Alfalfa was used as the mutagenesis material to explore the effective method of mutagenesis of alfalfa by HVEF through studying the synergistic effect of NaCl and HVEF.The experimental results showed that after the synergistic treatment of NaCl and HVEF，the survival rate was less than 50 percent of the control group，the stem length，root length，seedling height and fresh weight of alfalfa in the treatment group were lower than that in the control group，and the content of reactive oxygen species （ROS） was significantly higher than that in the control group.The experimental results showed that NaCl could improve the radiosensitivity of HVEF to alfalfa.

At the same time， in order to study the contribution of non-uniform electric field and ion wind in high voltage electric field， alfalfa was treated with high voltage electric field and shielding. The results showed that ion wind played an important role in the synergy of NaCl and high-voltage electric field.

In this research，a method was found to increase the fatality rate of alfalfa treated with NaCl combined with HVEF，which provided a new method for the mutagenesis of alfalfa and provided an experimental basis for the wider application of HVEF mutagenesis technology.

Key words NaCl;High voltage electric field;Alfalfa;Radiosensitivity

基金項目 国家自然科学基金项目（51767020）;内蒙古自然科学基金项目（2020MS01016，2019MS06025）。

作者简介 徐凯迪（1993—），男，安徽蚌埠人，硕士研究生，研究方向：电磁生物技术。通信作者，高级实验师，博士，从事环境生物物理方面的研究。

收稿日期 2020-07-23

紫花苜蓿是多年生豆科开花植物，它具有产量高、适应性强、草质优良、易于家畜消化等特点，是养殖业首选饲料，在畜牧业、农业耕作及生态环保等方面起着非常重要的作用[1]。目前，天然草地的劣变导致饲草产量降低、品质下降，草畜失衡的矛盾日益加剧，严重制约了畜牧业可持续发展。紫花苜蓿的诱变育种工作具有重要的意义。……