基于SSR分子标记的68份柚类种质资源亲缘关系分析

王旭 彭洁 朱延松 杨胜男 张晓楠 余洪 江东 梁大成

摘要 [目的]用17对SSR引物对68份柚类种质资源进行遗传背景研究，推演其亲缘关系，为更好地利用这68份材料提供参考。[方法]17对SSR引物进行PCR扩增;用 Power Marker V3.25軟件计算观测等位变异数（Na）、有效等位变异数（Ne）、观测杂合度（Ho）、期望杂合度（He）、香农多样性指数（I）和引物多态信息含量（PIC）;用 Ntsys2.0 计算材料间的遗传距离并进行聚类;用Structure2.3.4推导群体遗传结构。[结果]17对引物扩增出62条多态性条带，平均为3.647 0条。计算得出这62个多态性标记位点的平均等位变异数Na为3.470 6 ，平均有效等位变异数Ne为2.152 3 ，平均观测杂合度Ho为0.454 2 。平均期望杂合度He和香农多样性指数I分别为0.481 0和0.848 2，多态信息含量（PIC）平均值为0.419 8。在遗传距离0.36处将材料分成5个类群，结构分析中将材料分成4个组群，聚类分析和结构分析结果基本一致。[结论]所选材料具有丰富的遗传多样性，聚类和结构分析的结果能直观地了解这68份材料的亲缘关系，并对其中可能的同物异名品种进行筛选。

关键词 柚;SSR分子标记;亲缘关系

中图分类号 Q943  文献标识码 A

文章编号 0517-6611（2021）04-0100-04

doi：10.3969/j.issn.0517-6611.2021.04.027

Analysis of Genetic Relationship of 68 Pummelo Germplasm Resources Based on SSR Molecular Marker

WANG Xu1，PENG Jie2，ZHU Yan-song2 et al （1.School of Agriculture，Yangtze University，Jingzhou，Hubei 434025;2.Citrus Research Institute of Southwest University，Chongqing 400712）

Abstract [Objective] 17 pairs of SSR primers were used to study the genetic background of 68 pummelo germplasm resources and deduced their genetic relationship，so as to provide reference for better use of these 68 materials. [Method]17 PCR amplification was performed on SSR primers; Power Marker V3.25 software was used to calculate the observed allelic variance （Na），effective allelic variance （Ne），observed heterozygosity （Ho），expected heterozygosity （He ），Shannon diversity index （I） and primer polymorphism information content （PIC）; use Ntsys2.0 to calculate the genetic distance between materials and clustering; use Structure 2.3.4 to derive population genetic structure. [Result]The 17 pairs of primers amplified 62 polymorphic bands，with an average of 3.647 0 bands. It is calculated that the average allelic variance Na of the 62 polymorphic marker loci is 3.470 6，the average effective allelic variance Ne value is 2.152 3，and the average observed heterozygosity Ho value is 0.454 2. The average expected heterozygosity He value and Shannon diversity index I value are 0.481 0 and 0.848 2，respectively. The average PIC value of polymorphic information content is 0.419 8. The material was divided into 5 groups at the genetic distance of 0.36，and the material was divided into 4 groups in the structure analysis. The results of cluster analysis and structure analysis were basically the same. [Conclusion]The selected materials have rich genetic diversity，and the results of clustering and structural analysis can intuitively understand the genetic relationship of these 68 materials，and filter possible synonymous varieties among them.

Key words Pummelo;SSR molecular marker;Genetic relationship

基金项目 国家重点研发计划子课题“果树优质高效品种筛选及配套栽培技术研究”（2019YFD1001400）。

作者简介 王旭（1993—），男，重庆人，硕士研究生，研究方向：作物遗传育种。通信作者，江东，副研究员，硕士，硕士生导师，从事果树资源与遗传育种研究;梁大成，教授，博士，硕士生导师，从事湿地作物远缘嫁接技术研究。

收稿日期 2020-10-14;修回日期 2020-11-02

柚是柑橘属基本种之一，在柑橘中具有重要的生物学地位，同时在中国柑橘产业上也占有重要的经济地位[1]。……