硝酸镧对脐橙叶片2种膜透性物质含量及PPO活性的影响

2021-12-18 02:28:44张小丽陈璐高柱卢玉鹏王小玲
安徽农业科学 2021年23期

张小丽 陈璐 高柱 卢玉鹏 王小玲

摘要 以2年生纽荷尔脐橙嫁接苗为材料,通过喷施50、150、300 mg/L的硝酸镧[La(NO3)3]溶液,清水作为对照(CK),测定喷施前(0 h)和喷施后(2 、4 、8 、12、24 h)脐橙叶片中2种膜透性物质过氧化氢(H2O2)与丙二醛(MDA)含量及多酚氧化酶(PPO)活性的变化特性,阐明硝酸镧对脐橙叶片H2O2和MDA含量及PPO活性的影响。结果表明,硝酸镧处理后,脐橙叶片H2O2含量和PPO活性呈“先升高,后降低”的变化趋势。150 和300 mg/L硝酸镧处理不仅明显降低了脐橙叶片H2O2含量增加速度,延缓了H2O2含量峰值出现时间,而且明显提高了脐橙叶片PPO活性,其中150 mg/L的效果较好。另外,50 mg/L硝酸镧处理后脐橙叶片MDA含量峰值与CK出现的时间相同,处理后24 h,脐橙叶片MDA含量比CK降低了30%,而150 和300 mg/L处理则明显促进了脐橙叶片MDA含量的积累。因此,硝酸镧对脐橙叶片中H2O2、MDA和PPO的影响符合“低促高抑”的效应,适宜的处理浓度为50~150 mg/L。

关键词 硝酸镧;纽荷尔脐橙;过氧化氢;丙二醛;多酚氧化酶

中图分类号 S 666  文献标识码 A  文章编号 0517-6611(2021)23-0043-03

doi:10.3969/j.issn.0517-6611.2021.23.013

Effect of Lanthanum Nitrate on the Content of Two Osmoregulatory Substances and PPO  Activity in Navel Orange Leaves

ZHANG Xiao-li1,2, CHEN Lu1,2,GAO Zhu1  et  al

(1.Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330096; 2.Jinggangshan Institute of Biotechnology, Ji’an,Jiangxi  343016)

Abstract The 2-year-old ‘Newhall’ navel oranges grafted seedlings were sprayed with 50 , 150 , 300 mg/L La(NO3)3 solution, and water was used as control (CK). The content of hydrogen peroxide (H2O2), malondialdehyde (MDA) and the activity of polyphenol oxidase (PPO) in leaves were measured at different times (0, 2 , 4 , 8 , 12  and 24 h), to clarify the effect of lanthanum nitrate on the H2O2 and MDA content and PPO activity of navel orange leaves.The results showed that after be treated with La(NO3)3, the H2O2 content and PPO activity increased first and then decreased. The treatments of 150  and 300 mg/L not only significantly reduced the increase rate and delayed the peak time of H2O2 content, but also significantly improved the PPO activity, of which 150 mg/L had a better effect. After lanthanum nitrate treatment with 50 mg/L, the peak MDA content appeared at the same time as CK. 24 h after treatment, the MDA content decreased by 30% compared with CK, while 150  and 300 mg/L treatment significantly promoted the accumulation of MDA content. Therefore, the effect of lanthanum nitrate on H2O2, MDA and PPO in navel orange leaves conformed to the effect of “low promotion and high inhibition”, and the appropriate treatment concentration ranged from 50 to 150 mg/L.

Key words Lanthanum nitrate;‘Newhall’ navel oranges;H2O2;MDA;PPO

基金項目 国家自然科学基金项目(31760551)。

作者简介 张小丽(1995—),女,江西吉安人,助理研究员,硕士,从事园艺植物生物防治研究。

通信作者,研究员,博士,从事园艺植物高值利用研究。

收稿日期 2021-03-22

稀土元素是元素周期表中原子序数为55~71的镧系元素和与镧系密切相关的21号钪(Sc)、39号钇(Y)共17种元素的统称。稀土元素按其性质分为轻稀土和重稀土。我国稀土农用始于1972年,已有近50年的发展历程[1]。柑橘类水果种植面积和产量位居我国水果第一,其中脐橙是重要的柑橘类水果之一,栽培面积占柑橘总面积的18.6%[2]。……

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