补阳还五精简方对局灶性脑缺血大鼠脑组织APJ表达的影响

李峰等

摘要：目的 观察补阳还五精简方对局灶性脑缺血大鼠脑组织APJ蛋白和基因表达的影响，并探讨其作用机制。方法 采用大脑中动脉线栓法建立大鼠局灶性脑缺血模型，实验大鼠随机分为假手术组、模型组、补阳还五汤组和补阳还五精简方组，各给药组给予相应药物灌胃。分别于给药第3、7日Western blot、RT-PCR检测各组大鼠脑组织APJ蛋白和基因表达。结果 补阳还五精简方组和补阳还五汤组各时间点APJ蛋白和基因表达均高于模型组和假手术组（P<0.05），补阳还五精简方组与补阳还五汤组各时间点APJ蛋白表达无明显差异，但补阳还五精简方组APJ基因表达高于补阳还五汤组（P<0.05）。结论 补阳还五精简方可通过促进缺血后脑组织APJ的表达发挥对大鼠脑组织的神经保护和修复作用。

关键词：补阳还五精简方；局灶性脑缺血；APJ蛋白；大鼠

DOI：10.3969/j.issn.1005-5304.2015.09.014

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）09-0048-04

Effects of Simplified Recipe of Buyang Huanwu Decoction on Expression of APJ in Brain of Focal Cerebral Ischemia Rats LI Feng1， WANG Yu-hong2， SHE Yan1， YI Jian3， XIA Xiang-yi1， TAN Hu1， SHAO Le1， CAI Guang-xian3 （1.Hunan University of Chinese Medicine， Changsha 410208， China；2.Key Lab Breeding Base of Hunan Chinese Traditional Medicine Powder and Innovation Drugs Constructed by Provincial Government， Chansha 410208， China；3.Key Lab of Ministry of Education of Internal Medicine Major Diseases Prevention and Achievements Transformation， Hunan University of Chinese Medicine， Changsha 410007， China）

Abstract：Objective To observe the effects of simplified recipe of Buyang Huanwu Decoction on expression of APJ in the brain of local cerebral ischemia rats；To discuss its mechanism of action. Methods Focal cerebral ischemia rat models were established by middle cerebral arterial occlusion. The adult rats were randomly divided into sham-operation group， model group， Buyang Huanwu Decoction group and simplified recipe of Buyang Huanwu Decoction group. Administration groups were given relevant medicine for gavage. The expressions of APJ protein and APJ mRNA at different time points were detected by Western blot and RT-PCR. Results Compared with model group and sham-operation group， the expression of APJ protein and APJ mRNA at different time points in Buyang Huanwu Decoction group and simplified recipe of Buyang Huanwu Decoction group significantly increased （P<0.05）. The expression of APJ protein at different time points showed no difference between simplified recipe of Buyang Huanwu Decoction group and Buyang Huanwu

基金項目：国家重点基础研究发展计划（012CB723503）；湖南省高校创新平台开发基金项目（09k056）；湖南省科技厅项目（2013FJ4211）

通讯作者：蔡光先，E-mail：Cwlwj2008@126.com

Decoction group；while the expression of APJ mRNA in simplified recipe of Buyang Huanwu Decoction group was higher than Buyang Huanwu Decoction group （P<0.05）. Conclusion Simplified recipe of Buyang Huanwu Decoction plays a role in neural protection and restoration by promoting the expression of APJ in the brain of focal cerebral ischemia rats.

Key words：simplified recipe of Buyang Huanwu Decoction；focal cerebral ischemia；APJ；rats

脑缺血可造成大量脑组织损伤，其所致神经功能缺损根本原因是神经细胞损伤。脑缺血时会启动周围组织的修复和再生反应，诱导神经元再生。APJ蛋白是内源性神经肽Apelin的受体，Apelin/APJ能促进神经细胞增殖，可对抗脑缺血后的氧化应激损伤，保护脑组织[1]。补阳还五汤源自《医林改错》，对治疗缺血性中风及其后遗症疗效确切，对缺血性脑中风有显著疗效，补阳还五精简方取补阳还五汤之方义，基于益气祛瘀生新法精简方药而成。课题组前期研究发现，精简方有减小脑缺血后脑梗死面积及减轻脑水肿的作用[2]。本实验采用大脑中动脉线栓法复制大鼠局灶性脑缺血模型，探讨补阳还五精简方对局灶性脑缺血后APJ表达的影响，以期为治疗缺血性脑中风临床用药提供实验依据。……