心肌缺血型冠心病肾虚血瘀病证结合模型研究

周晟芳等

摘要：目的 探討手术方法建立心肌缺血型冠心病与肾虚血瘀病证结合模型的可行性。方法 采取惊吓、置于寒冷环境并皮下注射氢化可的松的方法制备非疾病肾虚血瘀证候模型，结扎冠状动脉前降支并注射氢化可的松建立心肌缺血型冠心病肾虚血瘀病证结合模型。实验大鼠分为肾虚血瘀组、病证结合组和正常组，每组5只。观察大鼠造模前后体温、体质量、呼吸频率、心率、全血黏度、卡松黏度。依据中医临床肾虚血瘀证诊断标准，对2种模型中医证候进行比较。结果 与正常组和本组造模前比较，肾虚血瘀组、病证结合组大鼠造模后均出现体温下降、呼吸频率增快（P<0.05）；与正常组比较，病证结合组、肾虚血瘀组体质量减轻或者增长缓慢、全血黏度及卡松黏度升高（P<0.05，P<0.01）；造模两组间全血黏度、心电图比较差异有统计学意义（P<0.05）。结论 2种模型在中医证候方面无明显差异，符合中医临床肾虚血瘀证的诊断标准。

关键词：心肌缺血型冠心病；动物模型；肾虚血瘀；病证结合；大鼠

DOI：10.3969/j.issn.1005-5304.2015.09.016

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）09-0056-04

Research on the Model of Combining Myocardial Ischemic Coronary Disease with Syndrome of Kidney Deficiency and Blood Stasis ZHOU Sheng-fang， LIU Ru-xiu， YIN Lin-lin， LUO He-wei， LI Hui， GUAN Xuan-ke （Guanganmen Hospital， China Academy of Chinese Medical Sciences， Beijing 100053， China）

Abstract：Objective To discuss the feasibility of establishing the model of combining myocardial ischemic coronary disease with syndrome of kidney deficiency and blood stasis by surgical method. Methods Non-disease of kidney deficiency and blood stasis syndrome model was built by taking the methods of fright， being placed in a cold environment and injection of hydrocortisone. The model of combining the myocardial ischemic coronary disease with the syndrome of kidney deficiency and blood stasis was established through ligating the left anterior descending branch of artery and injecting hydrocortisone. The rats were divided into kidney deficiency and blood stasis group， combination of disease and syndrome group， and normal group， 5 rats in each group. The temperature， weight， heart rate， breathing rate and whole blood viscosity， casson viscosity of the rats in the two groups before and after modeling were observed. According to TCM clinical diagnosis criteria of kidney deficiency and blood stasis syndrome， TCM syndrome characteristics of the two groups were compared. Results Compared with normal groups and before modeling， rat temperature dropped and breathing rate increased in kidney deficiency and blood stasis group， combination of disease and syndrome group （P<0.05）. Compared with normal group， rat weight decreased or grew slowly， and whole blood viscosity and casson viscosity increased （P<0.05， P<0.01）. There were statistical significant differences in whole blood viscosity and electrocardiogram between the two groups （P<0.05）. Conclusion There is no obvious difference between TCM syndrome characteristics of the two groups. They all meet the TCM clinical diagnosis criteria of kidney deficiency and blood stasis syndrome.

Key words：myocardial ischemic coronary disease；animal model；kidney deficiency and blood stasis；combination of disease and syndrome；rats

基金项目：国家科技支撑计划（2007BAI10B01-093）；北京市自然科学基金（7122153）；北京中医药“薪火传承3+3工程”（830104）

通讯作者：刘如秀，E-mail：Liuruxiu1@163.com

病证结合研究是中西医结合医学理论基础，建立动物病证结合模型是中医药现代研究取得突破性进展的关键。目前，中医药对心肌缺血型冠心病的实验研究大多局限于建立疾病模型，而忽视了动物模型的中医证候表现，难以全方位体现中医药治疗心肌缺血型冠心病的机制及特点。本研究通过手术方法建立心肌缺血型冠心病肾虚血瘀病证结合模型，并与非疾病肾虚血瘀证候模型相对照，通过比较其中医证候特点，探讨手术方法建立心肌缺血型冠心病肾虚血瘀病证结合模型的可行性。……