消痰散结方对MKN—45人胃癌裸鼠原位移植瘤细胞超微结构的影响

叶敏等

摘要：目的 观察消痰散结方对MKN-45人胃癌裸鼠原位移植瘤细胞超微结构的影响，探讨其治疗胃癌的作用机制。方法 采用MKN-45人胃癌细胞株建立裸鼠皮下瘤模型皮下传3代作为实验模型的瘤源，采用0B胶黏合法建立人胃癌裸鼠原位移植瘤模型。40只模型裸鼠分为空白对照组、模型组、消痰散结方组、替加氟组，每组10只。消痰散结方组给予消痰散结方灌胃，替加氟组给予替加氟灌胃，模型组给予生理盐水灌胃，空白对照组正常饮食喂养。干预6周后，测瘤质量，计算抑瘤率。透射电镜观察药物作用后移植瘤细胞超微结构。结果 消痰散结方组瘤质量明显低于空白对照组及模型组（P<0.05，P<0.01），其抑瘤率为46.2%；电镜观察可见胃癌细胞出现典型细胞凋亡的形态学改变。结论 消痰散结方具有诱导MKN-45人胃癌裸鼠原位移植瘤细胞凋亡的作用，提示诱导细胞凋亡是消痰散结方治疗胃癌的可能作用机制之一。

关键词：消痰散结方；胃癌；细胞超微结构；小鼠

DOI：10.3969/j.issn.1005-5304.2015.09.017

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）09-0060-03

Effects of Xiaotan Sanjie Decoction on Ultramicrostructure in MKN-45 Human Gastric Cancer Orthotopic Xenograft YE Min， SUN Da-zhi， XIU Li-juan， LU Ye， Instructor：WEI Pin-kang （Department of Traditional Chinese Medicine， Shanghai Changzheng Hospital， Second Military Medical University of PLA， Shanghai 200003， China）

Abstract：Objective To observe the effects of Xiaotan Sanjie Decoction on ultramicrostructure in MKN-45 human gastric cancer orthotopic xenograft；To discuss its mechanism for gastric cancer. Methods MKN-45 human gastric cancer cell line was used to establish subcutaneous tumor model in nude mice as an experimental model which passed three generations of tumor source. Then OB glue was used to establish human gastric cancer orthotopic xenograft model. 40 model nude mice were divided into control group， model group （received gavage with saline）， Xiaotan Sanjie Decoction group （received garage with Xiaotan Sanjie Decoction） and Tegafur group （received gavage with Tegafur）， 10 mice in each group. Mice in the control group were under normal diet， without medicine intervention. After 6 weeks of treatment intervention， tumor weight was detected， inhibitory rate was calculated， the ultrastructural changes of gastric cancer cells were detected through transmission electron microscopy. Results The tumor weight in Xiaotan Sanjie Decoction group was significantly lower than that in control group and model group （P<0.05，P<0.01）. The tumor inhibition rate was 46.2%. Under the electron microscope， MKN-45 gastric cancer cells strain changed to typical apoptosis. Conclusion Xiaotan Sanjie Decoction can lead to apoptosis effects on MKN-45 human gastric cancer orthotopic xenografts. All evidences indicate that inducing apoptosis may be one of the most important mechanisms of Xiaotan Sanjie Decoction in treating gastric cancer.

Key words：Xiaotan Sanjie Decoction；gastric cancer；cell ultrastructure；mice

基金項目：国家自然科学基金面上项目（81273910）

通訊作者：孙大志，E-mail：sunda-zhi@163.com

消痰散结方为我科魏品康教授临证40余年经验方，临床治疗胃癌疗效较好[1]。前期实验研究显示，消痰散结方可抑制MKN-45人胃癌裸鼠原位移植瘤的微卫星不稳定、在体外对结直肠癌HCT116细胞有抑制增殖、促进凋亡的作用[2-3]。本实验选取MKN-45人胃癌裸鼠原位移植瘤肿瘤细胞，通过透射电镜观察肿瘤细胞超微结构，进一步明确消痰散结方对胃癌干预的机理。

1 材料与方法

1.1 动物

SPF级雄性裸鼠40只，7周龄，体质量18～20 g，上海斯莱克实验动物有限公司，许可证号SYXK（沪） 2012-0002。实验动物饲养于中国科学院上海实验动物中心动物房的空气层流架内，饲养条件为SPF级。

1.2 细胞株

MKN-45人胃癌细胞株，中国科学院上海生命科学研究院细胞资源中心提供。……