Annexin A2在类风湿性关节炎滑膜组织中的表达

Annexin A2在类风湿性关节炎滑膜组织中的表达

陈扬*

( 浙江中医药大学第一临床医学院 杭州 311422)

摘 要目的：检测膜联蛋白（annexin）A2在类风湿性关节炎（rheumatoid arthritis，RA）滑膜组织中的表达，研究annexin A2与RA发生的相关性，期望能够在基因水平早期诊断和治疗RA，为明确RA的靶向性治疗提供帮助。 方法：采用real-time PCR技术检测annexin A2 mRNA在17例RA和15例骨性关节炎（osteoarthritis，OA）滑膜组织标本中的表达；采用苏木精-伊红染色法（hematoxylin-eosin staining，HE染色）对此32例滑膜组织标本进行了病理学观察，从炎性细胞浸润、纤维增生、滑膜细胞增生以及新生血管形成等4个方面加以评估。并比较两组的annexin A2 mRNA的相对表达量以及病理学观察评估结果。结果：annexin A2 mRNA在所有RA及OA滑膜组织样本中均有表达，但其在RA滑膜组织中的表达量显著高于OA滑膜组织（P<0.05）；HE染色显示RA组及OA组间炎性细胞浸润及纤维组织增生情况无显著性差异(P＞0.05)，但滑膜细胞增生及新生血管形成等病理改变存在显著性差异（P＜0.05），光学显微镜下在RA组可见滑膜细胞大量增生，且新生血管丰富，而这些在OA组少见。 结论：annexin A2与RA的发生关系密切，它的高表达可能在RA滑膜组织的病理改变中起到重要作用，有助于我们了解RA发生的分子生物学途径，进而为临床提供有针对性的治疗措施。

关键词类风湿性关节炎 annexin A2 real-time PCR HE染色

Expression of annexin A2 in rheumatoid arthritis synovial tissue

CHEN Yang*

(First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 311422, China)

ABSTRACTObjective: To detect the expression of annexin A2 in rheumatoid arthritis synovial tissue, and to study the relationship between annexin A2 and the occurrence of rheumatoid arthritis (RA). Methods: The expression of annexin A2 mRNA in the synovial tissue samples of 17 cases of RA and 15 cases of osteoarthritis (OA) patients was detected by real-time PCR and their pathological observation was performed by HE staining, and four indicators such as inflammatory cell infiltration, fibrosis, synovial cell proliferation and angiogenesis were assessed. The levels of the relative expression of annexin A2 mRNA and the assessment results of pathological observation were compared between two groups. Results: Annexin A2 mRNA was expressed in both RA and OA synovial tissue samples but the amount of annexin A2 mRNA expression was significantly higher in the RA group than in the OA group (P＜0.05). HE staining showed that there were no significant differences in inflammatory cell infiltration and fibrous tissue hyperplasia but there were significant differences in pathological changes such as proliferation of synovial cells and neovascularization (P＜0.05). A large amount of synovial cell proliferation and rich angiogenesis could be found in the RA group but less in the OA group under optical microscope. Conclusion: There is a close relationship between annexin A2 and the occurrence of RA, and the high expression of annexin A2 mRNA may play an important role in pathological changes of RA synovial tissue, which can help us understand a way of molecule biology for the occurrence of RA.

KEY WORDSrheumatoid arthritis; annexin A2; real-time PCR; HE staining

类风湿性关节炎（rheumatoid arthritis，RA）是一种以致残性关节病变为主的自身免疫性疾病，其病理特征主要表现为滑膜细胞异常增殖和炎性细胞浸润，累及周身关节滑膜炎及血管翳形成，严重时可导致滑膜增厚，形成侵袭性血管翳，侵蚀软骨与骨导致关节破坏，全世界约有1%的人患有此病，5～10年致残率高达60%[1]。

但其发病原因至今仍未明确，不过可以肯定的是RA是多因子、多步骤、多阶段的演进过程[2]。因此寻找与RA发生发展相关的敏感因子对其早期诊断及治疗有着重要意义。

annexin A2是一类钙依赖性膜磷脂结合蛋白，它参与细胞增殖、分化、凋亡和迁移等重要生命过程[3]。近年来国内外很多学者发现annexin A2的高表达与RA的发生密切相关[4-5]，但其在RA发生发展中的作用机制却鲜见报道。……