组织芯片技术在乳腺癌HER2 FISH检测中的可靠性分析

陈玉娟,张伟璇,朱桂璐,张 伟

(皖南医学院弋矶山医院病理科,芜湖 241001;*通讯作者,E-mail:weisky1981@163.com)



组织芯片技术在乳腺癌HER2 FISH检测中的可靠性分析

陈玉娟,张伟璇,朱桂璐,张 伟*

(皖南医学院弋矶山医院病理科,芜湖 241001;*通讯作者,E-mail:weisky1981@163.com)

目的 探讨乳腺癌组织芯片HER2 FISH检测结果的可靠性。 方法 收集192例乳腺癌病例,制成直径0.9 mm的组织芯片,进行HER2 FISH检测,对照常规石蜡切片的检测结果,进行统计学分析。 结果 组织芯片中HER2 FISH的阳性率为25.0%(48/192),常规石蜡切片的阳性率为26.0%(50/192),配对χ2检验显示两组之间差异无统计学意义(P>0.05)。有4例乳腺癌病例存在检测结果的不一致,其中常规石蜡切片检测为点状扩增病例中有3例组织芯片检测为阴性,常规石蜡切片检测为无扩增病例中有1例组织芯片检测为点状扩增。 结论 组织芯片技术在乳腺癌HER2 FISH检测中的可靠性强,但对于临床中乳腺癌病例建议使用常规石蜡切片进行HER2 FISH检测。

组织芯片; 乳腺癌; HER2; FISH

Analysis of the reliability of tissue microarrays in the research of HER2 FISH detection of breast cancer

CHEN Yujuan,ZHANG Weixuan,ZHU Guilu,ZHANG Wei*(DepartmentofPathology,YijishanHospital,WannanMedicalCollege,Wuhu241001,China;*Correspondingauthor,E-mail:weisky1981@163.com)Abstract：ObjectiveTo explore the reliability of tissue chip in the research of human breast cancer HER2 FISH detection.MethodsTissue microarray(TMA) with 0.9 mm in diameter tissue core was constructed from 192 cases of human breast cancer.HER2 was detected by FISH technique.At the same time the results of the tissue microarrays were compared with those of the routine paraffin section.ResultsThe positive rates in HER2 FISH detection of breast cancer were 25.0% and 26.0% by TMA and the routine paraffin section,respectively,and there was no statistical difference(P>0.05).But the results of 4 cases were different between the two methods.Three cases of dot expansion using the routine paraffin section were negative by TMA,and 1 case of dot expansion using TMA showed no expansion by the routine paraffin section.ConclusionTissue microarrays could be a reliable tool in the application of human breast cancer HER2 FISH detection,but it is better to use the routine paraffin section in the clinical.Key words: tissue microarrays; breast cancer; HER2; FISH

组织芯片(tissue chip,TC)即微缩组织切片,是将多量组织标本高密度排列成组织微阵列(tissue microarrays,TMA)的形式,具有体积小、信息量大的特点,一次实验即可获得大量结果。1998年Kononen等[1]首次在Nature杂志上报道了这一高通量生物芯片技术。与传统方法相比较,组织芯片具有实验条件一致性强、误差性小和准确性高的优点。但组织芯片本身制作过程冗繁,缺乏标准化流程。乳腺癌HER2 FISH检测已经成为一种常规病理工作,能否对多例乳腺癌标本利用组织芯片技术进行HER2 FISH检测是一项具有实际临床意义的工作。本研究参阅文献资料,对组织芯片的制作流程进行优化,制备出高质量的乳腺癌组织芯片,再探讨组织芯片技术在乳腺癌HER2 FISH检测中的可靠性,以期为临床病理工作提供帮助。