辣椒两种雄性不育系中可溶性蛋白质及可溶性糖的变化关系

黄 巍缪 武

（1.湖南省蔬菜研究所， 长沙 410125； 2. 湖南兴蔬种业有限公司， 长沙 410125；3.湖南湘研种业有限公司， 长沙 410100）

辣椒两种雄性不育系中可溶性蛋白质及可溶性糖的变化关系

黄 巍1,2缪 武3

（1.湖南省蔬菜研究所， 长沙 410125； 2. 湖南兴蔬种业有限公司， 长沙 410125；3.湖南湘研种业有限公司， 长沙 410100）

了解辣椒两种雄性不育系在花粉败育过程中可溶性蛋白质及可溶性糖的变化规律，以期为辣椒雄性不育系不育机理的研究提供参考。以辣椒细胞核雄性不育（GMS）G06-77-2AB及细胞核质互作雄性不育（CMS）系C06-77-2A为材料，用Lowry法和蒽酮法对蛋白质和糖进行测定并分析比较。结果表明：辣椒两种不同类型不育系花蕾发育的各个时期，可溶性蛋白质的含量不论是细胞核质互作不育系（C06-77-2A）还是细胞核雄性不育系（G06-77-2A）均明显低于其可育系（G06-77-2B），但是两种不育系之间没有明显规律可循。花蕾发育的各个时期，两种不同类型不育系可溶性糖含量均低于可育系，细胞核质互作不育系（C06-77-2A）明显低于细胞核雄性不育系（G06-77-2A）。两种雄性不育系在花蕾发育的各个时期，可溶性蛋白质和可溶性糖含量均低于可育系，两种不育系间无明显规律可循。

辣椒；CMS；GMS；可溶性蛋白质；可溶性糖

AbstractThe changes of the soluble protein and soluble sugar in the process of pollen abortion of the two-type male sterile lines were understood in this study so as to provide reference for the research of sterile mechanism of male sterile line in hot pepper. With a pepper genic male sterile (GMS) line G06-77-2 AB and a caryoplasm interaction male sterile (CMS) line C06-77-2 A as materials, Lowry method and anthrone method were used for the measure and comparison of the protein and sugar. The results showed that, in each development period of buds of the two types of pepper male sterile line, either the CMS line (C06-77-2A) or the GMS line (G06-77-2A) had the soluble protein content of buds signifcantly lower than its fertile line (G06-77-2B), but there was no clear rule to fnd between the two types of male sterile lines. And the soluble sugar content of buds of the two sterile lines was signifcantly lower than that of its fertile line, and the soluble sugar content of the CMS line (C06-77-2A) was signifcantly lower than that of the GMS line (G06-77-2A). The two types of male sterility had soluble protein and soluble sugar content lower than the fertile line at various stages of fower bud development, and there was no clear rule between the two sterile lines.

Key wordshot pepper; CMS; GMS; soluble protein; soluble sugar

蛋白质和糖是细胞组成中的重要物质，对小孢子的发生过程极为重要。刘齐元等[1]研究表明：雄性不育烟草花蕾中的可溶性蛋白质含量都比其相应保持系的低，而且随着花蕾的发育，这种差距还有扩大的趋势。邓明华等[2]研究表明：辣椒随着花蕾的发育，不育系中花蕾蛋白质的含量在大花蕾和中花蕾时期均低于小花蕾时期，而保持系花蕾中蛋白质的含量则在大花蕾时期明显高于中花蕾和小花蕾时期。……