降解菌短密木霉对土壤酶活性的影响

刘晴　董爱荣　邓世林　鲍彤　刘薇

摘要：为探究短密木霉以及短密木霉、大豆和咪唑乙烟酸相互作用对土壤蔗糖酶和过氧化氢酶活性的影响，本文采用3，5-二硝基水杨酸法、高锰酸钾滴定法分别测定土壤中蔗糖酶和过氧化氢酶的酶活。结果表明：在土壤中加入短密木霉，5～20 d中土壤蔗糖酶活性均呈现激活趋势，10 d时蔗糖酶活性最高为53.85 mg/kg，激活率达到169.94%;过氧化氢酶活性呈现出激活—抑制—恢复的趋势，5 d时土壤过氧化氢酶活性最高，为2.47 mg/g，激活率达5.71%，随后受到抑制，酶活性最低达2.30 mg/g，抑制率最高达到17.86%。种植大豆、加入短密木霉，再加入100 mg/kg的咪唑乙烟酸后，土壤蔗糖酶活性呈现激活—恢复—激活—抑制的趋势，10 d时土壤蔗糖酶活性最高，为60.09 mg/kg，激活率达201.21%，20 d时蔗糖酶活性被抑制，其活性为68.13 mg/kg，抑制率最高达62.43%;土壤过氧化氢酶活性呈现抑制—恢复—激活的趋势， 20 d时酶活性最低为2.43 mg/g，抑制率最高达到17.98%，40 d时酶活性最高，为2.70 mg/g，激活率最高达到9.46%。

关键词：短密木霉;蔗糖酶; 过氧化氢酶

中图分类号：S158.4文献标识码：A文章编号：1006-8023（2019）03-0009-04

Effects of Trichoderma brevicompactum on Soil Enzyme Activity

LIU Qing1， DENG Airong1*， DENG Shilin1， BAO Tong1， LIU Wei2

（1.College of Forestry， Northeast Forestry University， Harbin 150040; 2.Huludao Forestry Development Service Center， Huludao 125000）

Abstract：In order to investigate the effects of Trichoderma brevicompactum and the interaction of Trichoderma brevicompactum， soybean and imazethapyr on the activities of sucrase and catalase in soil， 3.5-dinitrosalicylic acid method and potassium permanganate titration were used to determine the activities of sucrose and catalase in soil respectively. The results showed that， when Trichoderma brevicompactum was added to the soil， in 5-20 days， sucrase activity showed a trend of activation with the highest activity of 53.85 mg/kg in 10 days and the activation rate of 169.94%. Catalase activity showed a trend of activation-inhibition-recovery. The highest catalase activity was 2.47 mg/g and the activation rate was 5.71%. Then it was inhibited. The lowest catalase activity was 2.30 mg/g and the highest inhibition rate was 17.86%. After planting soybean， adding Trichoderma brevicompactum and 100 mg/kg imazethapyr， soil sucrose activity showed the trend of activation-recovery-activation-inhibition. The highest sucrase activity was 60.09 mg/kg in 10 days， the activation rate was 201.21%， and the sucrase activity was in habited in 20 days with the highest sucrase activity of 68.12 mg/kg and the inhibition rate of 62.43%. Soil catalase activity showed a trend of inhibition-recovery-activation， the lowest activity was 2.43 mg/g in 20 days， the highest inhibition rate was 17.98%， the highest activity was 2.70 mg/g in 40 days， and the highest activation rate was 9.46%.

Keywords：Trichoderma brevicompactum; sucrase; catalase

0引言

土壤酶是土壤生态系统代谢的重要驱动力，土壤中进行化学和生物反应是在酶的催化下进行的。土壤微生物和土壤酶活性变化作为检测土壤质量变化的指标之一，反映了土壤生态系统中微生物参與物质转化和循环的能力，因此土壤酶受到人们越来越多的关注[1-2]。土壤酶参与土壤中所有的生化反应，在物质转化、能量代谢和污染土壤修复等过程中发挥着重要作用[3]。同时土壤酶作为一项生态毒理指标，常用于评价土壤健康及生态环境风险[4]。……