傣药双姜胃痛丸对小鼠的镇痛作用研究

李宝晶 王亭

【摘 要】 目的：研究双姜胃痛丸对小鼠的镇痛作用，初步考察其镇痛作用机制。方法：采用热板法和甲醛足底注射致痛法，以阿司匹林（0.2 g/kg）为阳性对照药，研究双姜胃痛丸高、中、低剂量组（3.00 g生药/kg、1.50 g生药/kg、0.75 g生药/kg）的镇痛作用。试剂盒法检测小鼠甲醛注射侧后足组织中肿瘤坏死因子（TNF）-α和前列腺素（PG）E2的含量及小鼠大脑组织中5-羟色胺（HT）和谷氨酸（Glu）的含量，PCR法检测小鼠甲醛注射后足组织中单核细胞趋化蛋白（MCP）-1的表达。结果：双姜胃痛丸中剂量能提高热刺激小鼠的痛阈值，高剂量能缩短甲醛致痛小鼠的Ⅰ相舔、咬足时间，低、中、高剂量组均能显著缩短Ⅱ相舔、咬足时间，并明显降低小鼠甲醛注射后足组织中PGE2含量、TNF-α含量和MCP-1 mRNA表达水平，但对小鼠大脑组织中5-HT和Glu含量无明显影响。结论：双姜胃痛丸具有明显的镇痛作用，其镇痛机制与减轻局部炎症因子和致痛介质释放相关。

【关键词】 双姜胃痛丸;甲醛致痛;前列腺素E2;肿瘤坏死因子-α;单核细胞趋化蛋白-1

【中图分类号】R285.5   【文献标志码】 A    【文章编号】1007-8517（2020）12-0013-05

Abstract：Objective To investigate analgesic effect of Shuangjiang Weitong Pill（SJWT）in mice. Methods After oral administration of SJWT（0.75，1.50，3.00 g/kg）for 7 days，the hot plate test and formalin-induced pain model were used to assess the analgesic effect of SJWT. After formalin test，prostaglandin（PG）E2 and tumor necrosis factor（TNF）-α concentrations of the injected paw tissue，5-hydroxytryptamine（HT）and glutamic acid concentrations of brain tissue were detected by ELISA，and of the injected paw tissue monocyte chemoattractant protein（MCP）-1 mRNA expression was determined by Q-PCR. Results SJWT at 1.50 g/kg prolonged the latency time induced by hot stimuli，SJWT at 3.00 g/kg reduced total time of licking，biting the injected paw in phase Ⅰ. SJWT at each dosage reduced total time of licking，biting the injected paw in phase Ⅱ，and reduced TNF-α and PGE2 concentrations and the expression of MCP-1 of the injected paw tissue. Conclusions SJWT could exert analgesic effect on mice，and the potential mechanism might be related to decrease the production and release of proinflammatory factors and nociception factors.

Keywords：Shuangjiang Weitong Pill;Formalin–Induced Mice Pain Model;Prostaglandin（PG）E2; Tumor Necrosis Factor（TNF）-α; Monocyte Chemoattractant Protein（MCP）-1

雙姜胃痛丸为傣药成药，其组方被列为傣医的经典药方。全方由毫命（姜黄Curcuma longa Linn.）、补累（紫色姜Zingiber purpureum Rosc.）、波波罕（地不容Stephania epigaea H.S. Lo）、罕好帕（石菖蒲Acorus tatarinowii Schott）、反帕嘎[苦菜子Brassica integrifolia（West）O. E. Schulz]组成。其中姜黄、紫色姜性温热，入风、土塔，具有通气消胀、活血止痛之功，为主药;石菖蒲性热，入风、水、土塔，主治脘腹胀痛，不思饮食，为辅助药;地不容、苦菜子味苦，性凉，既有凉血止痛之功，还可抑制主药之燥性，为抑制药[1]。在功能主治方面，傣医认为双姜胃痛丸能“通塞勒，塞拢，补塔铃。……