调控萼脊兰类黄酮生物合成的MYB转录因子挖掘分析

蒋素华　牛苏燕　袁秀云　梁芳　王喜蒙　崔波

摘  要：本研究通过生物信息学方法，挖掘萼脊兰中可能调控类黄酮生物合成的MYB转录因子。从萼脊兰转录组数据库中筛选MYB转录因子，获得MYB的完整ORF，并对其蛋白理化性质、基序、功能注释、系统进化、表达特性等进行分析，并预测其功能。结果表明：在萼脊兰转录组水平上共鉴定出27个具有完整阅读框的MYB转录因子基因，萼脊兰MYB转录因子TRINITY_DN38485_c0_g4和TRINITY_DN38485_c0_g1基因均在花朵和叶片中表达上调，再根据与蝴蝶兰的系统进化关系与功能分析，预测这2个转录因子可能通过黄酮途径完成类黄酮生物合成。因此，MYB转录因子的研究对从分子水平上研究和调节类黄酮的合成具有重要意义，转录因子的应用是类黄酮生物合成基因工程中的新途径。

关键词：萼脊兰;MYB转录因子;次生代谢

中图分类号：Q946.8      文献标识码：A

Mining Analysis of MYB Transcription Factors Related to Flavonoid Biosynthesis of Sedirea japonica

JIANG Suhua1， NIU Suyan1， YUAN Xiuyun1， LIANG Fang1， WANG Ximeng2， CUI Bo1*

1. Biological Engineering Research Center， Zhengzhou Normal University， Zhengzhou， Henan 450044， China; 2. College of Life Sciences， Zhengzhou University， Zhengzhou， Henan 450002， China

Abstract： MYB transcription factors that may regulate the biosynthesis of the flavonoids in Sedirea japonica were detected using bioinformatics methods. The complete ORF of MYB was obtained， and the physicochemical properties， motif， functional annotation， phylogenetic evolution， and expression characteristics of its proteins were analyzed， and its functions were also predicted. A total of 27 MYB transcription factor genes with complete reading frames were identified at the transcriptome level of S. japonica. MYB transcription factors TRINITY_DN38485_c0_g4 and TRINITY_ DN38485_c0_g1 were up-regulated in flowers and leaves. Then according to the phylogenetic relationship with phalaenopsis and functional analysis，it is predicted that the two transcription factors were involved in flavonoid biosynthesis through the flavonoid pathway. Therefore， the study of MYB transcription factor is of great significance to study and regulate the synthesis of flavonoids at the molecular level. The application of transcription factors is a new approach in the genetic engineering of flavonoid biosynthesis， the application of transcription factors is a new approach in the genetic engineering of flavonoid biosynthesis.

Keywords： S. japonica; MYB transcription factors; secondary metabolism

DOI： 10.3969/j.issn.1000-2561.2021.03.003

轉录因子（transcription factor）是一群能与基因5? 端上游特定序列专一性结合，从而保证目的基因以特定强度在特定时间与空间表达的蛋白质分子。根据转录因子的作用特点可分为两类：第一类为普遍转录因子;第二类转录因子为组织细胞特异性转录因子[1-2]。人们在植物中已经找到了很多调控黄酮类次生代谢的特异性转录因子基因，主要包括编码MYB、MYC、bZIP、WD40蛋白和锌指蛋白等基因家族[3-4]。MYB基因家族广泛存在于植物中，是植物中最大的转录因子基因家族之一。在类黄酮生物合成过程中，MYB转录因子扮演着重要角色，可调控与类黄酮合成相关的酶基因的表达，从而有效地调控类黄酮物质的生物合成。……