白杨素对心肌缺血再灌注损伤大鼠心肌Bax和Bcl-2表达的影响

罗斌 田芳芳 田俊斌 吕俊林 马磊 吕建瑞

摘要 目的：研究白楊素对心肌缺血再灌注损伤（MI/RI）大鼠的作用，同时观察其对心肌凋亡相关蛋白Bax和Bcl-2表达的影响。方法：SPF级SD大鼠60只适应性喂养2周后，按体质量大小依次分为假手术组、模型组、阳性药物雷米普利组、白杨素低、中和高剂量组，每组10例，疗程2周，后采用可逆左冠状动脉前降支结扎法建立MI/RI模型，假手术组只穿线但不结扎。手术后收集血液检测血清肌酸激酶同功酶（CK-MB）、乳酸脱氢酶（LDH）、白细胞介素（Interleukin，IL）-6、超氧化物歧化酶（SOD）和丙二醛（MDA）含量，TTC法评估心肌梗死面积，HE染色观察心肌病理学，Western Blot检测心肌Bax和Bcl-2蛋白表达特点。结果：与假手术组比较，模型组心肌梗死面积明显增加（P<0.05），心肌纤维断裂，与模型组比较，白杨素3个剂量组和雷米普利组心肌梗死面积降低（P<0.05），心肌细胞形态改善;与假手术组比较，模型组血清CK-MB、LDH、IL-6和MDA增加（P<0.05），SOD降低（P<0.05），与模型组比较，白杨素3个剂量组和雷米普利组CK-MB、LDH、IL-6和MDA降低（P<0.05），SOD升高（P<0.05）;与假手术组比较，模型组心肌Bax蛋白表达明显增加（P<0.05），Bcl-2表达降低（P<0.05），与模型组比较，白杨素3个剂量组和雷米普利组Bax蛋白表达降低（P<0.05），Bcl-2上调（P<0.05）。结论：白杨素对MI/RI大鼠血清心肌酶CK-MB和LDH，炎性因子IL-6，氧化和抗氧化指标MDA和SOD均有调节作用，同时可降低心肌梗死面积，其作用机制可能与白杨素能够调节Bcl-2和Bax蛋白表达有关。

关键词 白杨素;大鼠;心肌缺血再灌注;心肌细胞;凋亡

Effect of Chrysin on Bax and Bcl-2 in Rats with Myocardial Ischemia Reperfusion Injury

Luo Bin1，Tian Fangfang2，Tian Junbin1，LYU Junlin1，MA Lei1，LYU Jianrui1

（1 The Second Affiliated Hospital of Xi′an Jiaotong University，Xi′an 710004，China; 2 Department of Anesthesiology，Tangdu Hospital，Second Affiliated Air Force Military Medical University，Xi′an 710038，China）

Abstract Objective：To study the therapeutic effects of chrysin on MI/RI rats，and to observe the proteins expression of Bax and Bcl-2.Methods：According to weight，A total of 60 SD rats after adaptive feeding for 2 weeks were divided into a sham operation group，a model group，a positive drug ramipril group，chrysin low，medium and high dose groups，n=10.The course of treatment was 2 weeks.Later，the MI/RI model was established by reversible left anterior descending coronary artery ligation.The sham operation group only threaded but not ligated.Later，the MI/RI model was established by reversible left anterior descending coronary artery ligation.The sham operation group only threaded but not ligated.After the operation，blood was collected for serum creatine kinase isoenzyme（CK-MB），lactate dehydrogenase（LDH），interleukin（IL）-6，superoxide dismutase（SOD）and malondialdehyde（MDA）content.TTC method was used to evaluate myocardial infarction area，HE staining was used to observe myocardial pathology.Western Blot was used to detect myocardial Bax and Bcl-2 protein expression characteristics.Results：Compared with the sham group，the infarct size in the model group was increased（P<0.05），the myocardial fibers were broken，compared with the model group，the myocardial infarct size was improved in the 3 dose groups of Chrysin and Ramipril group（P<0.05）.Cardiomyocyte morphology improved; compared with the sham group，serum CK-MB，LDH，IL-6 and MDA increased in the model group（P<0.05），SOD decreased（P<0.05）.com

pared with the model group，CK-MB，LDH，IL-6 and MDA in the 3 doses of chrysin and ramipril group decreased（P<0.05），and SOD decreased（P<0.05）; Compared with the sham operation group，the expression of Bax protein in the myocardium of the model group was significantly increased（P<0.05），and the expression of Bcl-2 was decreased（P<0.05）.com

pared with the model group，the three-dose group of chrysin and expression Bax protein in Puri group was reduced（P<0.05），and Bcl-2 was up-regulated（P<0.05）.Conclusion：Chrysin has a regulatory effect on serum myocardial enzymes CK-MB and LDH，inflammatory factor IL-6，oxidation and antioxidant indicators MDA and SOD in MI/RI rats，and can reduce the area of myocardial infarction.Its mechanism of action may be related to that of chrysin regulating Bcl-2 and Bax protein expression.

Keywords Chrysin; Rat; Myocardial ischemia-reperfusion injury（MI/RI）; Cardiomyocyte; Apoptosis

中图分类号：R242;R285.5文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.01.014

心肌缺血再灌注损伤（Myocardial Ischemia-reperfusion Injury，MI/RI）是冠心病死亡的主要原因，患者突发急性心肌梗死后，可诱发心肌细胞大量死亡，进而诱发心肌细胞增生、凋亡以及胶原疤痕等一系列病理反应[1]。一般而言，在早期心肌重塑过程中心脏有一定能力能够维持自身结构完整和心功能正常，但患者發生持续性心肌梗死后会导致心肌结构改变和心功能异常，这也是导致患者死亡的根本因素[2-3]。目前在临床中并没有特效药物可抑制或逆转心肌结构重塑，因此延缓心肌重塑是当前研究热点[4]。白杨素是黄酮类化合物的一种，其在蜂胶中含量较高，近年来研究发现，白杨素具有多种药理活性，具有抗病……