河西地区赤霞珠葡萄果实发育期糖代谢及相关酶活性的变化

边彩燕 姜寒玉 朱永永 陈超 熊春蓉 赵贵宾

摘要：以采自甘肃紫轩葡萄酒业葡萄园的酿酒葡萄赤霞珠为试验材料，对果实中葡萄糖、果糖、蔗糖含量及糖代谢相关酶活性进行测定。结果表明，随着赤霞珠葡萄果实的生长，蔗糖、葡萄糖、果糖含量显著上升。不同生长发育阶段，赤霞珠果实中酸性转化酶、中性转化酶、蔗糖合成酶、蔗糖磷酸合成酶活力不同。相关性分析表明，整个发育期的葡萄糖、果糖、蔗糖与酸性转化酶活性存在显著正相关，R值分别为0.789、0.726、0.719;葡萄糖、果糖、蔗糖与中性转化酶活性、蔗糖磷酸合成酶、蔗糖合成酶活性均表现为极显著正相关。

关键词：赤霞珠;糖代谢;转化酶;合成酶

中图分类号：S663.1    文献标志码：A    文章编号：1001-1463（2021）06-0042-07

doi：10.3969/j.issn.1001-1463.2021.06.011

Changes in Sugar Metabolism and Related Enzyme Activities of Cabernet sauvignon Grape Fruits during Development in Hexi Region

BIAN Caiyan 1， JIANG Hanyu 2， ZHU Yongyong 1， CHEN Chao 1， XIONG Chunrong 1， ZHAO Guibin 1

（1. Gansu Agricultural Technology Extension Station， Lanzhou Gansu 730000， China; 2. College of Physical Science and Technology， Nanjing Normal University， Nanjing Jiangsu 210046， China）

Abstract：Cabernet sauvignon was selected from the vineyard of Gansu Zixuan Wine Industry as the experimental material， the contents of glucose， fructose， sucrose and the activities of enzymes related to sugar metabolism in fruits were determined. The results showed that the content of sucrose， glucose and fructose increased significantly with the growth of Cabernet sauvignon grapes. The activities of acid invertase， neutral invertase， sucrose synthase and sucrose phosphate synthase were different in Cabernet sauvignon fruits at different growth and development stages. Correlation analysis showed that glucose， fructose， sucrose and activity of acidic invertase activity in the whole development period were significantly positively correlated， R values of 0.789， 0.726 and 0.719， respectively. The activities of neutral invertase， sucrose phosphate synthase and sucrose synthase were significantly positively correlated with glucose， fructose and sucrose.

Key words：Cabernet sauvignon;Sugar metabolism;Invertase;Synthetase

葡萄在我國属于六大水果之一， 在果树种植中有着举足轻重的作用[1 - 2 ]。甘肃省河西走廊是我国重要的葡萄种植基地，历史悠久，已形成了独具特色的葡萄酒文化，基本形成了武威、张掖、嘉峪关、酒泉四大产区。该区域内的酿酒葡萄，具有粒大、色泽好、气味浓郁等特点。葡萄浆果中包含多种糖类，如葡萄糖、果糖及蔗糖，含量一般为15%～25%。糖不单单是口感的代名词，也决定着浆果维生素、芳香物质的含量，所以，含糖量是评价果实品质的重要指标之一。目前已经发现，植物体内糖代谢直接影响着植物的生长发育，以强化植物生育及品质为根本目的，围绕糖代谢过程进行探析，具有一定的现实意义[3 ]。葡萄果实品质的高低往往对应着糖种类的多少及含量的大小，可以说，糖分的种类、含量决定着果实本身的营养价值、风味口感等各类品质[4 - 6 ]。……