扶正消积胶囊对胃癌细胞BGC-823的增殖抑制及凋亡的影响

薛刚 刘杰 许利军 石建 凌博凡

摘要 目的：研究扶正消积胶囊在体外对人胃癌细胞BGC-823增殖抑制及凋亡的影响。方法：MTT实验检测扶正消积胶囊对BGC-823细胞增殖的影响;流式细胞仪检测其对BGC-823细胞凋亡的影响;Real Time-PCR、Western blotting实验检测其凋亡相关基因表达。结果：与空白组比较，BGC-823细胞的增殖能被扶正消积胶囊显著抑制（给药24 h），此抑制率随给药剂量的增加而上升，在剂量为5 mg/ mL时，其抑制率达86.71%，且这种抑制呈剂量依赖关系。Annexin V/PI双染法联合流式细胞仪结果显示扶正消积胶囊能诱导BGC-823细胞凋亡，BGC-823细胞早期凋亡率随给药剂量的增加，在给药剂量分别为2.5 mg/mL、5 mg/mL时，BGC-823细胞早期凋亡率分别达到15.76%、38.25%;Western blotting、Real Time-PCR结果表明，Procaspase-3、9蛋白酶表达随剂量的增加而明显减小，Bcl-2、Bcl-xl、Survivin 3种基因表达随给药剂量的增加而下降，Bax基因表达则相反。结论：扶正消积胶囊能通过激活caspase级联反应抑制胃癌细胞增殖并诱导其凋亡。

关键词 扶正消积胶囊;BGC-823胃癌细胞;肿瘤;增殖;凋亡

Abstract Objective：To investigate the influence of Fuzheng Xiaoji Capsule on the proliferation inhibition of human gastric cancer cell BGC-823 and its apoptosis.Methods：Effects of Fuzheng Xiaoji Capsule on proliferation of BGC-823 cells were investigated with MTT assay.Flow cytometry was used to detect the cell apoptosis while the effect of apoptosis related gene of Fuzheng Xiaoji capsule on BGC-823 cell was measured by real time-PCR and Western blotting.Results：MTT assay showed that the BGC-823 cells were inhibited by Fuzheng Xiaoji Capsule compared with control group，after treated on 24 h.The inhibition rate increases with the increase of dose，which is dose dependent.At the dose of 5 mg/mL，the inhibition rate reached 86.71%（P<0.05）.Flow cytometry demonstrated that Fuzheng Xiaoji Capsule could induce the apoptosis of BGC-823 cells.The early apoptosis rate of BGC-823 cells increased with the dose of the drug.At the dose of 2.5 mg/mL and 5 mg/mL，respectively，the early apoptosis of BGC-823 cells reached 15.76% and 38.25% respectively.By the results of Western blotting and real time-PCR，the expression of caspase-3、caspase-9 was significantly lower as the dose increased.Also，it could reduce the activity of Bcl-2，Bcl-xl，Survivin and increase the activity of Bax.Conclusion：Fuzheng Xiaoji Capsule could inhibit the proliferation of BGC-823 cells and induce their apoptosis.The possible mechanism was through activation of caspase cascade reaction.

Keywords Fuzheng Xiaoji Capsule; BGC-823 human gastric cancer cell; Cancer; Proliferation; Apoptosis

中图分类号：R273文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.10.005

胃癌是世界范圍内最常见的恶性肿瘤之一，也是我国常见的恶性肿瘤之一，在我国西北以及东部沿海地区胃癌发病率高居首位[1-3]。手术仍是目前治疗胃癌的主要手段，术后化疗在胃癌治疗中占重要地位，不良反应是大多数胃癌患者无法完成既定化疗疗程的主要原因。近年来，中医药在治疗胃癌方面取得重大进展，尤其是在改善临床症状、提高生命质量、延长生存时间等方面疗效肯定[4-5]。临床工作中，中药与化疗相结合既能提高化疗效果，亦能减轻化疗不良反应。……