液相色谱一串联质谱法测定血清中尿素含量

唐强

摘 要：针对传统血清中尿素含量测定方法操作困难，准确度低的问题，提出液相色谱一串联质谱法测定尿素中血清含量。分别对两种浓度水平下水清中尿素浓度定值，精准测试盲样中尿素含量。全面分析了影响血清中尿素含量的因素，并根据分析结果对其不确定度进行评价。结果表明：液相色谱一串联质谱法测定血清中尿素含量相对偏差（RSD）均小于1%。对两种浓度水平的血清进行測定，不确定度分别为1.2%和0.84%，这就证明本文所建立的方法测量血清中尿素含量比较准确，且原理比较可靠，定量比较准确，前处理简单，可以当做血清中尿素含量测定的候选参考方法。

关键词：血清;尿素;同位素稀释质谱法;液相色谱

中图分类号：R446.1 文献标识码：A     文章编号：1001-5922（2021）10-0033-04

Determination of Urea in Serum by Liquid Chromatography Tandem Mass Spectrometry

Tang Qiang

（Hunan College of Foreign Studies， Changsha 410116， China）

Abstract：Aiming at the problems of difficult operation and low accuracy of traditional methods for the determination of urea in serum， a liquid chromatography tandem mass spectrometry method was proposed to determine the content of urea in serum. Determine the concentration of urea in the water at two concentration levels， and accurately test the urea content in the blind sample. The factors affecting the content of urea in the serum were comprehensively analyzed， and the uncertainty was evaluated based on the analysis results. The results showed that the relative deviation （RSD） of urea content in serum determined by liquid chromatography tandem mass spectrometry was less than 1%. The uncertainty of urea in two concentrations of serum was 1.2% and 0.84% respectively， which prove that the method established in this paper is more accurate in measuring serum urea content， and the principle is more reliable， the quantification is more accurate， and the pretreatment is simple， it can be used as a candidate reference method for the determination of urea content in serum.

Key words：serum; urea; isotope dilution mass spectrometry; liquid chromatographypounds

尿素是人体内氨基酸分解代谢的产物，受肝脏功能和蛋白质摄入量影响。在临床医学中，尿素代谢是反映肾功能情况的非特异性性指标，也是在正常人普通饮食的情况下，一项较为敏感的指标。因此，准确检测血清中尿素含量是临床医学中，非常重要的一项研究。目前较为常见的血清中尿素测定方法有脲酶速率法[1]、离子电导法[2]、二乙酰肟法[3]，这些方法虽然能够检测出血清中尿素的含量，但精准度还需要进一步提高。针对以上方法存在的问题，张鹏[4]提出气相色谱/同位素稀释质谱法，为病人血清提供了一个高度准确的测定工具。但该方法存在测试过程困难，衍生化试剂使用条件严格，衍生化反应所需时间长，步骤繁琐的问题，使得尿素的测定出现新的难题。……