紫丹银屑颗粒对银屑病模型小鼠的HaCaT细胞周期和血清IL－2、TNF－α的影响

紫丹银屑颗粒对银屑病模型小鼠的HaCaT细胞周期和血清IL-2、TNF-α的影响

李伟妮1，李忠原1,崔文2，王平3，孙虎3，李百开1

(1.烟台东诚大洋制药有限公司，山东 烟台 265500；2.青岛大学附属医院，山东 青岛 266003；

3.山东省中医药研究院，山东 济南 250014)

摘要：目的探讨紫丹银屑颗粒对银屑病样小鼠模型的治疗作用和作用机制，为临床应用提供参考。方法将试验动物随机分为正常对照组、模型对照组、紫丹银屑高、中、低剂量组及阳性对照组，每组10只，灌胃给药。采用流式细胞分析仪分析紫丹银屑颗粒对HaCaT细胞周期的影响(在96孔板上依次设定紫丹银屑颗粒2、4、8、16、32 g·L-1组，维A酸片8 g·L-1组，空白细胞组)；采用动物体内实验，观察紫丹银屑颗粒对银屑病小鼠模型血清中白介素-2、肿瘤坏死因子含量的影响。结果体外试验显示，与模型组比较，紫丹银屑颗粒高、中剂量组细胞增殖数量显著减少，有显著性差异(P<0.05)；体内试验显示，与模型组比较，紫丹银屑颗粒高、中剂量组S期细胞数量显著减少，有显著性差异(P<0.05)。结论紫丹银屑颗粒能够显著抑制人永生化表皮细胞增殖，并显著降低模型动物血清白介素-2、肿瘤坏死因子的含量，这可能是紫丹银屑颗粒治疗银屑病的作用机制之一。

关键词：紫丹银屑颗粒；HaCaT细胞；白介素-2；肿瘤坏死因子；小鼠

作者简介：李伟妮，女，研究方向：中药学，E-mail:liweini@163.com

通讯作者：王平，女，副研究员，研究方向：中药药理，Tel:0531-82949812,E-mail:wangpingjinan@163.com

中图分类号：R965文献标识码：A

Effect on the HaCaT cell generation cycle and serum IL-2，TNF-α of

psoriasis sample model mice treating by Zidanyinxie Particle

LIWei-ni1，LIZhong-yuan1,CUIWen2，WANGPing3,SUNHu3，LIBai-kai1

(1.YantaiDongchengDayangPharmaceuticalCo.,Ltd.,Yantai265500，China；2.TheAffiliated

HospitalofQingdaoUniversity,Qingdao266003，China；3.DepartmentofPharmacology,

ShandongAcademyofChineseMedicine，Jinan250014，China)

Abstract：ObjectiveTo investigate the effect and mechanism of Zidanyinxie Particle for mice psoriasis sample.MethodsAll the mice were randomly divided into normal control group,model group,Zidanyinxie Particle high dosage group,Zidanyinxie Particle middle dosage group，Zidanyinxie Particle small dosage group,and positive control group,each group had 10 mice，gavaged.Prepared serum containing drug,concentration was Zidanyinxei Particle 2,4,8,16,32 g·L-1,Tretinoin Tablets 8 g·L-1.In vitro test，to detect the effect of Zidanyinxie Particle in HaCaT cell generation cycle;In vivo test,after giving relevant drugs，to observe serum IL-2 and TNF-α of vaginal epithelial pathological model mice.ResultsIn vitro experiments,compared with model control group，cell proliferation was significantly reduce in the Zidanyinxie Particle high dosage group,Zidanyinxie Particle middle dosage group,P<0.05.In vivo studies,compared with normal control group，the number of mitotic vaginal epithelial cells were significantly increased in model group,P<0.05,and compared with model control group，the number of mitotic vaginal epithelial cells were significantly decreased in Zidanyinxie Particle high dosage group,Zidanyinxie Particle middle dosage group,P<0.05.ConclusionZidanyinxie Particle could inhibited the proliferation of HaCaT cell and reduced the Serum IL-2,TNF-α content of mice model significantly，this may be one of the mechanisms of Zidanyinxie Particle in treating psoriasis.

Key words:Zidanyinxie Particle;HaCaT cell;IL-2;TNF-α;Mice

银屑病是一种皮肤科常见的易反复发作的疾病，被世界卫生组织定为十大顽疾之一。临床表现为周身或者局部皮肤白色鳞屑，病理学表现为表皮细胞过度增殖及异常分化，炎症细胞浸润，真皮血管增生[1，2]。银屑病根治困难，容易复发，病程迁延日久，肌肤失养，摧残身体，给患者带来极大的心理和生理负担，临床迫切需要开发相应的治疗药物。……