17β—E2联合顺铂对人卵巢癌细胞裸鼠移植瘤的影响

邹路遥　林国志

[摘要] 目的 研究17β-E2单用或联合顺铂（DDP）作用于人卵巢癌细胞株 HO-8910裸鼠移植瘤的影响。方法 建立人卵巢癌HO-8910裸鼠皮下移植瘤模型，随机分成6组：A组（生理盐水组）、B组（低剂量17β-E2）、C组（高剂量17β-E2）、D组（顺铂5 mg/kg）、E组（低剂量17β-E2+顺铂）、F组（高剂量17β-E2+顺铂），每组5只。观察各组人卵巢癌裸鼠移植瘤的生长情况、各组裸鼠的体重改变。 结果 A组、B组、C组、D组、E组、F组裸鼠体重均增加，B组、C组、D组、E组、F组治疗后裸鼠体重均比A组体重轻，但各组差异无统计学意义。B组、C组、D组、E组、F组移植瘤体积、瘤重均明显减少，其中E组、F组治疗后比单独用药体积变化明显，两组相比较，F组移植瘤体积、瘤重均更小。 结论 高剂量17β-E2联合顺铂更具有抑制人卵巢癌裸鼠移植瘤生长的作用。

[关键词] 17β-E2；顺铂；卵巢癌；移植瘤

[中图分类号] R737.31 [文献标识码] A [文章编号] 1673-9701（2018）04-0039-04

[Abstract] Objective To study the effects of 17β-E2 alone or combined with cisplatin on the growth of transplanted tumor of human ovarian cancer cell line HO-8910 in nude mice. Methods Subcutaneously transplanted tumor model of human ovarian cancer HO-8910 was established and randomly divided into 6 groups： group A（normal saline group）， group B（low dose 17β-E2）， group C（high dose 17β-E2）， group D（cisplatin 5 mg/kg）， group E （low dose 17β-E2+cisplatin）， and group F（high dose 17β-E2+cisplatin）， with 5 mice in each group. The growth of transplanted tumor of human ovarian cancer in nude mice in each group was observed， and the changes of body weight of nude mice in each group were observed. Results The body weight of nude mice in group A， group B， group C， group D， group E and group F was increased， and the body weight of nude mice in group B， C， D， E and F after treatment was all lighter than that in group A， but there was no statistical significance in each group. In group B， group C， group D， group E and group F， the volume of transplanted tumor and the weight of tumor were decreased significantly， and the volume change in group E and group F after treatment was more significant than that of administration alone. Compared between the two groups， the tumor volume and tumor weight in group F were both lower. Conclusion High dose of 17β-E2 combined with cisplatin has a better inhibitory effect on the growth of transplanted tumor of human ovarian cancer in nude mice.

[Key words] 17β-E2； Cisplatin； Ovarian cancer； Transplanted tumor

卵巢癌是婦科常见的恶性肿瘤，因为早期无明显症状，大部分女性查体发现卵巢癌时，病情已处于中晚期阶段，超过5年生存率的卵巢癌患者仅有40%[1，2]。手术加术后辅助化疗是主要治疗手段。患者术后卵巢去势导致雌激素水平低下而出现绝经综合征，严重影响患者的术后生命质量。激素替代治疗（Hormone replacement therapy，HRT）是改善卵巢去势妇女雌激素低下症状、提高生命质量的最佳方法。但上皮性卵巢癌患者术后应用HRT能否促进肿瘤复发尚存在争议。本文通过建立人卵巢癌HO-8910裸鼠皮下移植瘤模型，观察不同剂量17β-雌二醇（17β-E2）单独或与DDP联合应用对人卵巢癌裸鼠皮下移植瘤的作用，从而为卵巢肿瘤防治研究提供新方法和开创新途径。……