大孔树脂分离纯化柞树叶总黄酮及其抗氧化活性研究

王林美 岳冬梅 孙永欣 马淑慧 张波 李树英

摘要 [目的]研究柞树叶总黄酮的纯化工艺及抗氧化活性。[方法]采用静态吸附与解析的方法，通过比较6种大孔树脂的吸附和解析性能，优选适宜的大孔树脂，并优化适合分离柞树叶总黄酮的解析条件。以VC为对照，对其还原力、DPPH 自由基（DPPH·）和超氧阴离子自由基（O2-·）的清除能力进行研究。[结果]D101型大孔树脂纯化柞树叶总黄酮吸附与洗脱效果最好，其纯化最佳工艺条件为粗提液浓度0.55 mg/mL、上样流速1.5 mL/min、洗脱剂40%乙醇（V/V）、洗脱流速1.5 mL/min，柞树叶总黄酮纯度可达65.5%;分离纯化后柞树叶总黄酮具有良好的总还原能力以及清除DPPH自由基和抗超氧阴离子自由基活性，其总还原力大于VC的还原力，当质量浓度为0.03 mg/mL时，对DPPH自由基和抗超氧阴离子自由基的清除率分别为85.9%和65.9%。[结论]D101大孔树脂可用于柞树叶总黄酮的分离纯化，柞树叶总黄酮具有较强的抗氧化活性。

关键词 柞树叶;总黄酮;大孔树脂;分离纯化;抗氧化活性

中图分类号 R 285  文献标识码 A

文章編号 0517-6611（2021）23-0192-06

doi：10.3969/j.issn.0517-6611.2021.23.054

Study on Separation and Purification of Total Flavonoids from Tussah Leaves by Macroporous Resin and Its Antioxidant Activity

WANG Lin-mei，YUE Dong-mei，SUN Yong-xin et al

（Liaoning Ocean and Fisheries Science Research Institute，Dalian，Liaoning 116023）

Abstract [Objective]To study the purification technology and antioxidant activity of total flavonoids from tussah leaves.[Method]The suitable macroporous resin and the suitable desorption conditions for total flavonoids separation from tussah leaves were optimized by comparing the adsorption and desorption properties of six kinds of macroporous resin through static test.The reducing power，the scavenging ability of DPPH radical （DPPH·） and superoxide anion radical （O2-·） were studied，as VC was control .[Result]The adsorption and elution effect of total flavonoids from tussah leaves by D101 macroporous resin was the best.The purity of total flavonoids in tussah leaves could reach 65.5% with the optimum purification conditions： crude extract concentration of 0.55 mg/mL，sample flow rate of 1.5 mL/min，elution agent of 40% ethanol （V/V），elution flow rate of 1.5 mL/min.After separation and purification，the total flavonoids of tussah leaves had good total reducing ability and scavenging DPPH free radical and anti-superoxide anion free radical activity，and its total reducing power was greater than that of VC.When the mass concentration was 0.03 mg/mL，the scavenging rates of DPPH free radical and anti-superoxide anion radical were 85.9% and 65.9%，respectively.[Conclusion] D101 macroporous resin can be used for the separation and purification of total flavonoids from tussah leaves，and the total flavonoids from tussah leaves have strong antioxidant activity.

Key words Tussah leaves;Total flavonoids;Macroporous resin;Separation and purification;Antioxidant activity

基金项目 现代农业产业技术体系建设专项（CARS-18）。

作者简介 王林美（1966—），女，辽宁大连人，副研究员，从事蚕桑资源综合利用 研究。通信作者，研究员，从事柞蚕资源综合利用研究。

收稿日期 2021-04-06

柞树为壳斗科栎属植物的总称，是多年生阔叶植物，我国主要分布在东北和华北地区，其中辽宁省柞树林有182万hm2，占林地面积的14.7%，资源广泛，野外自然生长，产地天然无污染[1]。柞树叶主要作为柞蚕饲料而被利用，研究发现柞……