夏枯草种子萌发及其幼苗生长对干旱胁迫的响应

2017-08-13 08:12:49王媛媛彭亮王晶杨冰月杜疏炀胡本祥
安徽农业科学 2017年7期

王媛媛 彭亮 王晶 杨冰月 杜疏炀 胡本祥

摘要 [目的]探讨干旱胁迫对夏枯草种子及其幼苗生长的影响。[方法]采用不同浓度的聚乙二醇(PEG-6000)对夏枯草种子进行处理,测定种子发芽率、发芽指数、发芽势以及幼苗的主根长、株高、鲜重、干重等指标。[结果]随干旱强度的增加,不同浓度的PEG干旱胁迫对夏枯草种子萌发及幼苗生长产生不同程度的影响。[结论]低浓度(2.5%~5.0%)PEG胁迫作用小,高浓度(10.0%~15.0%)PEG胁迫作用大;10.0%浓度PEG促使地下根长生长;对于地上部分,低浓度抑制不明显,高浓度各指标均产生显著抑制。

关键词 夏枯草;聚乙二醇;干旱胁迫;种子萌发;幼苗生长

中图分类号 S567.23+9 文献标识码 A 文章编号 0517-6611(2017)07-0104-03

Seed Germination and Seedling Growth of Prunella vulgaris L. in Response to Drought Stress

WANG Yuan-yuan, PENG Liang*, WANG Jing, HU Ben-xiang* et al

(College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046)

Abstract [Objective] To discuss the effects of drought stress on germination of Prunella vulgaris L. seeds and seedling growth. [Method] By using different concentrations of polyethylene glycol(PEG-6000), seed germination rate (Gr), germination index (GI) , germination potential (Gp)and seedling root length, plant height, fresh weight and dry weight indicators were determined. [Result] The results indicated that different PEG concentrations had different effects on the germination of P. vulgaris seeds and seedlings growth with the increase of drought intensity. [Conclusion] Low concentrations(2.5%-5.0%) of PEG have the little effects on P. vulgaris seeds, while high concentrations(10.0%-15.0%) have a large effects, 10.0% concentration promotes the growth of underground part, for the overground part, low concentration suppression is not obvious, high concentrations of various indexes significantly inhibit.

Key words Prunella vulgaris L.;PEG-6000;Drought stress;Seed germination;Seedling growth

夏枯草(Prunella vulgaris L.)為唇形科夏枯草屬的多年生草本植物,果穗入药,主产于安徽、江西、江苏、浙江、山东等地,具有清肝泻火、消肿散结的功效,主治眩晕、目赤肿痛、瘰疬、瘿瘤等症[1]。由于其重要的药用价值和养生保健作用,市场需求量也在逐步增加。近年来,夏枯草人工栽培工作已在各地开展起来,但在实际大田生产过程中发现夏枯草的出苗率及幼苗生长极易受到土壤干旱的危害[2]。种子萌发期的生长情况不仅会影响自身正常的生长,还会影响到植株下一代的繁育,因此水分胁迫是种子萌发出苗情况最直观的表现,种子萌发期是研究植物抗旱性的重要时期[3]。研究表明,不同程度的干旱胁迫对种子发芽的影响表现不同[4-5]。该试验以夏枯草种子为材料,采用不同浓度的聚乙二醇(PEG-6000)模拟干旱胁迫环境,研究夏枯草种子萌发及幼苗生长特性的变化情况,为夏枯草种子的育种和种植栽培提供参考。……

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